Prasad Vure, Dorle Avinash Kumar
Department of Pharmaceutical Sciences, Nagpur University, India.
J Ethnopharmacol. 2006 Aug 11;107(1):38-47. doi: 10.1016/j.jep.2006.02.006. Epub 2006 Mar 20.
Formulation containing neomycin and ghee was evaluated for wound-healing potential on different experimental models of wounds in rats. The rats were divided into six groups of group 1 as control, group 2 as treated with neomycin only, group 3 as treated only with ghee, group 4 treated with F-1 formulation containing ghee 40% and neomycin 0.5%, group 5 treated with F-2 formulation containing ghee 50% and neomycin 0.5% and group 6 treated with F-3 formulation containing ghee and ointment base in all two wound models, each group consisting of six rats. Wound contraction ability in excision wound model was measured at different time intervals and study was continued until wound is completely healed. Tensile strength was measured in 10-day-old incision wound and quantitative estimation of hydroxy proline content in the healed tissue was determined in 10-day-old excision wound. Histological studies were done on 10-day-old sections of regenerated tissue of incision wound. F-2 formulation containing ghee 50% and neomycin 0.5% showed statistically significant response, in terms of wound contracting ability, wound closure time, period of epithelization, tensile strength of the wound, regeneration of tissues at wound site when compared with the control group and these results were comparable to those of a reference neomycin ointment.
评估了含有新霉素和酥油的制剂对大鼠不同伤口实验模型的伤口愈合潜力。将大鼠分为六组，第1组为对照组，第2组仅用新霉素治疗，第3组仅用酥油治疗，第4组用含有40%酥油和0.5%新霉素的F-1制剂治疗，第5组用含有50%酥油和0.5%新霉素的F-2制剂治疗，第6组用含有酥油和软膏基质的F-3制剂治疗。在两种伤口模型中，每组由六只大鼠组成。在切除伤口模型中，在不同时间间隔测量伤口收缩能力，并持续研究直至伤口完全愈合。在10日龄的切口伤口中测量拉伸强度，并在10日龄的切除伤口中测定愈合组织中羟脯氨酸含量的定量估计。对10日龄的切口伤口再生组织切片进行组织学研究。与对照组相比，含有50%酥油和0.5%新霉素的F-2制剂在伤口收缩能力、伤口闭合时间、上皮形成期、伤口拉伸强度、伤口部位组织再生方面显示出统计学上的显著反应，这些结果与参考新霉素软膏的结果相当。
