Butel J S, Melnick J L, Rapp F
Department of Virology and Epidemiology, Baylor University College of Medicine, Houston, Texas.
J Bacteriol. 1966 Aug;92(2):433-8. doi: 10.1128/jb.92.2.433-438.1966.
Butel, Janet S. (Baylor University College of Medicine, Houston, Tex.), Joseph L. Melnick, and Fred Rapp. Detection of biologically active adenovirions unable to plaque in human cells. J. Bacteriol. 92:433-438. 1966.-Plaque formation in green monkey kidney (GMK) cells by a defective simian virus 40-adenovirus 7 "hybrid" population (PARA-adenovirus 7) was enhanced by the addition of excess adenovirions. Adenovirus types 2, 7, and 12 were capable of providing enhancement, although none of these viruses gives rise to plaques in simian cells in the absence of PARA (particle aiding replication of adenovirus). Near maximal enhancement of the PARA plaque titer on simian cells was obtained with input multiplicities ranging from 0.02 to 0.14 plaque-forming units (PFU) of helper adenovirus per GMK cell. The PFU of helper adenoviruses tested (types 2, 7, and 12) were measured in the most sensitive assay system, human kidney cells. This input corresponded to three to nine helper virus particles per GMK cell. The majority of particles capable of enhancing plaque formation by PARA banded at a density of 1.34 in CsCl. Adenoviruses inactivated by heat or ultraviolet light were not capable of enhancing plaque formation by PARA. Highest titers were obtained when PARA and helper adenovirus were inoculated simultaneously. Inoculation of the helper adenovirus 24 hr prior to the inoculation of PARA resulted in the formation of only 50% as many plaques, and no enhanced plaques developed when the adenovirus preceded PARA by 48 hr. Conversely, the addition of adenovirus 48 hr after the inoculation of PARA initiated 56% as many plaques as simultaneous inoculation; 4% of the enhanced plaques still formed when helper virus was added as late as 5 days after inoculation of PARA. These results suggest that adenovirus particles unable to plaque on human or monkey kidney cells are nevertheless capable of interacting with PARA in simian cells, thereby facilitating replication of both particles.
布特尔，珍妮特·S.（得克萨斯州休斯顿贝勒医学院），约瑟夫·L.梅尔尼克，以及弗雷德·拉普。检测在人细胞中无法形成噬斑的具有生物活性的腺病毒颗粒。《细菌学杂志》92:433 - 438。1966年。——通过添加过量腺病毒颗粒，缺陷型猿猴病毒40 - 腺病毒7“杂交”群体（PARA - 腺病毒7）在绿猴肾（GMK）细胞中形成噬斑的能力得到增强。2型、7型和12型腺病毒能够提供增强作用，尽管在没有PARA（辅助腺病毒颗粒复制的粒子）的情况下，这些病毒在猿猴细胞中都不会形成噬斑。当每个GMK细胞输入的辅助腺病毒的噬斑形成单位（PFU）范围为0.02至0.14时，猿猴细胞上PARA噬斑滴度接近最大增强。所测试的辅助腺病毒（2型、7型和12型）的PFU是在最敏感的检测系统人肾细胞中测量的。这种输入量相当于每个GMK细胞有三到九个辅助病毒颗粒。大多数能够增强PARA噬斑形成的颗粒在CsCl中的密度为1.34。经热或紫外线灭活的腺病毒不能增强PARA的噬斑形成。当PARA和辅助腺病毒同时接种时获得最高滴度。在接种PARA前24小时接种辅助腺病毒，形成的噬斑数量仅为同时接种时的50%，当腺病毒比PARA提前48小时接种时，没有形成增强噬斑。相反，在接种PARA后48小时添加腺病毒，形成的噬斑数量为同时接种时的56%；当辅助病毒在接种PARA后5天这么晚才添加时，仍有4%的增强噬斑形成。这些结果表明，在人或猴肾细胞上无法形成噬斑的腺病毒颗粒仍然能够在猿猴细胞中与PARA相互作用，从而促进两种颗粒的复制。
