Kingsmore Stephen F
National Center for Genome Resources, Santa Fe, New Mexico 87505, USA.
Nat Rev Drug Discov. 2006 Apr;5(4):310-20. doi: 10.1038/nrd2006.
The ability to measure the abundance of many proteins precisely and simultaneously in experimental samples is an important, recent advance for static and dynamic, as well as descriptive and predictive, biological research. The value of multiplexed protein measurement is being established in applications such as comprehensive proteomic surveys, studies of protein networks and pathways, validation of genomic discoveries and clinical biomarker development. As standards do not yet exist that bridge all of these applications, the current recommended best practice for validation of results is to approach study design in an iterative process and to integrate data from several measurement technologies. This review describes current and emerging multiplexed protein measurement technologies and their applications, and discusses the remaining challenges in this field.
在实验样本中精确且同时测量多种蛋白质丰度的能力，是近期生物学研究在静态与动态、描述性与预测性方面取得的一项重要进展。多重蛋白质测量在诸如全面蛋白质组学调查、蛋白质网络和信号通路研究、基因组发现验证以及临床生物标志物开发等应用中，其价值正不断得到确立。由于尚未存在适用于所有这些应用的标准，目前推荐的结果验证最佳做法是通过迭代过程进行研究设计，并整合来自多种测量技术的数据。本文综述了当前及新兴的多重蛋白质测量技术及其应用，并讨论了该领域尚存的挑战。
