Department of Molecular Biology, Zoecon Corporation, 975 California Avenue, Palo Alto, California 94303.
Proc Natl Acad Sci U S A. 1981 Nov;78(11):7043-6. doi: 10.1073/pnas.78.11.7043.
The cytoplasmic male sterility (CMS) trait of at least one line of Vicia faba plants is always associated with the presence of high molecular weight double-stranded RNA in the leaf tissue extracts. Subcellular fractions of leaf tissue from CMS and fertile maintainer plants were initially analyzed in an attempt to locate, identify, and characterize the genetic material involved with the sterility trait. This CMS-associated high molecular weight RNA was found only in the cytosol of the "447" male sterile line of V.faba plants and could not be isolated from the recurrent parent (maintainer), from lines that had been fertility-restored, or from lines that had reverted from the sterile condition. We have been able to move the CMS-associated RNA from donor to fertile host plants through a dodder bridge. These hots not only contain the RNA but now exhibit a male sterile phenotype, as detected by visual examination of the flower, the pollen, morphological characteristics, and pollen staining ability.
至少有一种蚕豆植物的细胞质雄性不育（CMS）性状总是与叶片组织提取物中存在高分子量双链 RNA 有关。最初分析了 CMS 和可育维持系植物的亚细胞组织部分，试图定位、鉴定和表征与不育性状相关的遗传物质。这种 CMS 相关的高分子量 RNA 仅存在于蚕豆“447”雄性不育系的细胞质中，无法从轮回亲本（维持系）、已恢复育性的系或已从不育状态恢复的系中分离出来。我们已经能够通过菟丝子桥将 CMS 相关的 RNA 从供体转移到可育的宿主植物中。这些植物不仅含有 RNA，而且现在表现出雄性不育表型，通过对花、花粉、形态特征和花粉染色能力的视觉检查即可检测到。
