Helmke Brian P, Minerick Adrienne R
Department of Biomedical Engineering, University of Virginia, P.O. Box 800759, Charlottesville, VA 22908, USA.
Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6419-24. doi: 10.1073/pnas.0507304103. Epub 2006 Apr 17.
Nanotechnology-based materials are beginning to emerge as promising platforms for biomedical analysis, but measurement and control at the cell-chip interface remain challenging. This idea served as the basis for discussion in a focus group at the recent National Academies Keck Futures Initiative. In this Perspective, we first outline recent advances and limitations in measuring nanoscale mechanical, biochemical, and electrical interactions at the interface between biomaterials and living cells. Second, we present emerging experimental and conceptual platforms for probing living cells with nanotechnology-based tools in a microfluidic chip. Finally, we explore future directions and critical needs for engineering the cell-chip interface to create an integrated system capable of high-resolution analysis and control of cellular physiology.
基于纳米技术的材料正开始成为生物医学分析的有前景的平台，但细胞与芯片界面的测量和控制仍然具有挑战性。这一观点是最近美国国家科学院凯克未来计划焦点小组讨论的基础。在这篇观点文章中，我们首先概述了在测量生物材料与活细胞界面处的纳米级机械、生化和电相互作用方面的最新进展和局限性。其次，我们介绍了用于在微流控芯片中使用基于纳米技术的工具探测活细胞的新兴实验和概念平台。最后，我们探讨了工程化细胞与芯片界面以创建能够对细胞生理学进行高分辨率分析和控制的集成系统的未来方向和关键需求。
