Franz G, Loukeris T G, Dialektaki G, Thompson C R, Savakis C
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece.
Proc Natl Acad Sci U S A. 1994 May 24;91(11):4746-50. doi: 10.1073/pnas.91.11.4746.
Elements related to the Tc1-like Minos mobile element have been cloned from Drosophila hydei and sequenced. Southern blot and sequence analyses show that (i) the elements are actively transposing in the Drosophila hydei germ line, (ii) they are characterized by a striking degree of sequence and size homogeneity, and (iii) like Tc1, they insert at a TA dinucleotide that is probably duplicated during the process. The nucleotide sequences of two elements, Minos-2 and Minos-3, differ at only one position from each other and contain two nonoverlapping open reading frames that are separated by a putative 60-nucleotide intron. The amino-terminal part of the Minos putative transposase shows sequence similarity to the paired DNA-binding domain. Forced transcription of a modified Minos element that was introduced into the Drosophila melanogaster germ line by P element-mediated transformation resulted in the production of accurately spliced polyadenylylated RNA molecules. It is proposed that Minos-2 and/or Minos-3 may encode an active transposase containing an amino-terminal DNA-binding domain that is distantly related to the paired DNA-binding domain.
已从海德氏果蝇中克隆并测序了与类Tc1的minos移动元件相关的元件。Southern印迹和序列分析表明：（i）这些元件在海德氏果蝇种系中活跃转座；（ii）它们具有显著程度的序列和大小同质性；（iii）与Tc1一样，它们插入到一个TA二核苷酸处，该二核苷酸在这个过程中可能会被复制。两个元件Minos - 2和Minos - 3的核苷酸序列仅在一个位置上彼此不同，并且包含两个不重叠的开放阅读框，它们被一个推测的60个核苷酸的内含子隔开。minos推测的转座酶的氨基末端部分与配对的DNA结合结构域具有序列相似性。通过P元件介导的转化引入黑腹果蝇种系的修饰minos元件的强制转录导致了准确剪接的多聚腺苷酸化RNA分子的产生。有人提出，Minos - 2和/或Minos - 3可能编码一种活性转座酶，该转座酶含有一个与配对的DNA结合结构域有远缘关系的氨基末端DNA结合结构域。
