Xie Cheng-Hui, Yokota Akira
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
Int J Syst Evol Microbiol. 2006 May;56(Pt 5):1117-1121. doi: 10.1099/ijs.0.64115-0.
Strain DY(T), which was isolated from garden soil in Japan, was subjected to a polyphasic taxonomic study. Sequence analysis of the 16S rRNA gene and the GyrB protein revealed that the closest relative of strain DYT was [Flavobacterium] ferrugineum Sickles and Shaw 1934, with 94.8 and 90.1 % similarity, respectively. The two strains had similar chemotaxonomic characteristics, with menaquinone 7 as the major quinone system, 47.2-48.9 mol% DNA G+C content and 15 : 0 iso, 15 : 1 iso, 17 : 0 iso 3-OH and summed feature 3 as the major fatty acids. Based on genotypic and phenotypic characteristics, [Flavobacterium] ferrugineum IAM 15098T could be clearly differentiated from other members of the genus Flavobacterium. Strain DYT and [Flavobacterium] ferrugineum IAM 15098T could be easily distinguished from neighbouring taxa by morphological features (non-motile, non-gliding and non-filamentous single cells). Therefore, it is proposed that [Flavobacterium] ferrugineum IAM 15098T and strain DYT represent two separate species of a new genus, Terrimonas gen. nov., with the names Terrimonas ferruginea comb. nov. (type species; type strain IAM 15098T=ATCC 13524T) and Terrimonas lutea sp. nov. (type strain DYT=IAM 15284T=CCTCC AB205006T), respectively.
从日本花园土壤中分离出的菌株DY(T)，进行了多相分类学研究。16S rRNA基因和GyrB蛋白的序列分析表明，菌株DYT的最接近亲缘种是1934年的铁锈色黄杆菌（[Flavobacterium] ferrugineum）Sickles和Shaw，相似性分别为94.8%和90.1%。这两个菌株具有相似的化学分类特征，以甲基萘醌7作为主要醌系统，DNA G+C含量为47.2 - 48.9 mol%，以iso-15 : 0、iso-15 : 1、iso-17 : 0 3-OH和特征性脂肪酸总和3作为主要脂肪酸。基于基因型和表型特征，铁锈色黄杆菌（[Flavobacterium] ferrugineum）IAM 15098T可以与黄杆菌属的其他成员明显区分开来。菌株DYT和铁锈色黄杆菌（[Flavobacterium] ferrugineum）IAM 15098T可以通过形态特征（不运动、不滑动且不成丝状的单细胞）很容易地与邻近分类单元区分开来。因此，建议将铁锈色黄杆菌（[Flavobacterium] ferrugineum）IAM 15098T和菌株DYT分别代表一个新属——土栖菌属（Terrimonas gen. nov.）的两个独立物种，分别命名为铁锈色土栖菌（Terrimonas ferruginea comb. nov.）（模式种；模式菌株IAM 15098T = ATCC 13524T）和黄色土栖菌（Terrimonas lutea sp. nov.）（模式菌株DYT = IAM 15284T = CCTCC AB205006T）。
