College of Rural Revitalization, Jiangsu Open University, Nanjing, China.
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
Curr Microbiol. 2024 Nov 2;81(12):438. doi: 10.1007/s00284-024-03967-4.
A Gram-stain-negative bacterium, designated strain LY-5, was isolated from an activated sludge sample collected from a river in Yancheng city, Jiangsu province, China. Cells of strain LY-5, were strictly aerobic, non-motile and rod-shaped. Growth occurred at 15-37 °C (optimum, 30 °C), at pH 5.0-9.0 (optimum 7.0) and at 0-3% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on 16S rRNA gene and genome sequences indicated that strain LY-5 formed a distinct phyletic branch within the family Chitinophagaceae, with closest relatives being members of the genera Phnomibacter, Aurantisolimonas, and Chitinophaga, sharing 88.5-90.3% sequence similarity. Moreover, the average amino acid identity (AAI) between strain LY-5 and its closed phylogenetic neighbors was below 70%, indicating it belongs to a novel genus. The predominant cellular fatty acids of LY-5 were iso-C, iso-C G and summed feature 3 (C ω7c and/or C ω6c), and the only isoprenoid quinone was menaquinone-7 (MK-7). The major polar lipids identified in strain LY-5 comprised phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids. The genome size of strain LY-5 was 4.8 Mbp with a G + C content of 38.9%. Based on the evidence presented in this study, strain LY-5 represents a novel species of a new genus in the family Chitinophagaceae, for which the name Polluticaenibacter yanchengensis gen. nov., sp. nov. (Type strain LY-5 = CCTCC AB 2023260 = KCTC 102218) is proposed.
一株革兰氏阴性细菌，命名为LY-5 菌株，从中国江苏省盐城市一条河流中的活性污泥样本中分离得到。LY-5 菌株的细胞为严格需氧、非运动和杆状。生长发生在 15-37°C（最佳温度 30°C），pH 值为 5.0-9.0（最佳 pH 值为 7.0），0-3%（w/v）NaCl（最佳 0%）。基于 16S rRNA 基因和基因组序列的系统发育分析表明，LY-5 菌株在几丁质噬菌科内形成一个独特的分支，与亲缘关系最近的是 Phnomibacter、Aurantisolimonas 和 Chitinophaga 属的成员，序列相似度为 88.5-90.3%。此外，LY-5 菌株与其密切相关的系统发育邻居之间的平均氨基酸同一性（AAI）低于 70%，表明它属于一个新属。LY-5 的主要细胞脂肪酸为 iso-C、iso-C G 和总和特征 3（C ω7c 和/或 C ω6c），唯一的异戊烯醌是甲萘醌-7（MK-7）。LY-5 菌株中鉴定出的主要极性脂质包括磷脂酰乙醇胺、两种未鉴定的氨基脂和五种未鉴定的脂质。LY-5 菌株的基因组大小为 4.8 Mbp，G+C 含量为 38.9%。基于本研究提供的证据，LY-5 菌株代表几丁质噬菌科一个新属的新种，建议将其命名为 Polluticaenibacter yanchengensis gen. nov.，sp. nov.（模式菌株 LY-5=CCTCC AB 2023260=KCTC 102218）。
