Institut de Science des Materiaux de Mulhouse, CNRS LRC 7228, Université de Haute Alsace, 68057, Mulhouse cedex, France.
J Mater Sci Mater Med. 2010 Mar;21(3):939-46. doi: 10.1007/s10856-009-3950-7. Epub 2009 Dec 10.
Osteosarcoma-derived cell lines (SaOs-2, MG63) have recently been shown to deform their nucleus considerably in response to surface topography. Such a deformation had not been described previously. Here we present results on additional cell lines, including cancerous (OHS4, U2OS), immortalized (F/STRO-1(+)A and FHSO6) and healthy cells (HOP). The cancerous cells were found to deform extensively, the immortalized cells showed small deformations, whereas the healthy cells showed deformation only at short incubation times. These results suggest a strong link between the malignant transformation of cells and the state of the cytoskeletal network. We propose mechanisms to explain the deformation in which the cytoskeleton either pushes down on the nucleus during spreading or pulls it down upon adhesion to the pillars.
成骨肉瘤衍生的细胞系（SaOs-2、MG63）最近被证明会在响应表面形貌时显著改变其细胞核的形状。这种变形以前尚未被描述过。在这里，我们呈现了关于其他细胞系的结果，包括癌细胞（OHS4、U2OS）、永生化细胞（F/STRO-1(+)A 和 FHSO6）和正常细胞（HOP）。发现癌细胞广泛变形，永生化细胞显示出较小的变形，而正常细胞仅在短孵育时间内显示出变形。这些结果表明细胞的恶性转化与细胞骨架网络的状态之间存在很强的联系。我们提出了一些机制来解释这种变形，即细胞骨架在铺展过程中向下推挤细胞核，或者在与柱子黏附时向下拉动细胞核。
