Borrelli Francesca, Capasso Francesco, Capasso Raffaele, Ascione Valeria, Aviello Gabriella, Longo Rocco, Izzo Angelo A
Department of Experimental Pharmacology, University of Naples Federico II, Italy.
Br J Pharmacol. 2006 Jun;148(4):553-60. doi: 10.1038/sj.bjp.0706740. Epub 2006 Apr 24.
Clinical studies suggest that the Ayurvedic plant Boswellia serrata may be effective in reducing diarrhoea in patients with inflammatory bowel disease. In the present study, we evaluated the effect of a Boswellia serrata gum resin extract (BSE) on intestinal motility and diarrhoea in rodents. BSE depressed electrically-, acetylcholine-, and barium chloride-induced contractions in the isolated guinea-pig ileum, being more potent in inhibiting the contractions induced by acetylcholine and barium chloride. The inhibitory effect of BSE on acetylcholine-induced contractions was reduced by the L-type Ca(2+) channel blockers verapamil and nifedipine, but not by the sarcoplasmic reticulum Ca(2+)-ATPase inhibitor cyclopiazonic acid, by the phosphodiesterase type IV inhibitor rolipram or by the lipoxygenase inhibitor zileuton. 3-acetyl-11-keto-beta-boswellic acid, one of the main active ingredients of B. serrata, inhibited acetylcholine-induced contractions. BSE inhibited upper gastrointestinal transit in croton oil-treated mice as well as castor oil-induced diarrhoea. However, BSE did not affect intestinal motility in control mice, both in the small and in the large intestine. It is concluded that BSE directly inhibits intestinal motility with a mechanism involving L-type Ca(2+) channels. BSE prevents diarrhoea and normalizes intestinal motility in pathophysiological states without slowing the rate of transit in control animals. These results could explain, at least in part, the clinical efficacy of this Ayurvedic remedy in reducing diarrhoea in patients with inflammatory bowel disease.
临床研究表明，阿育吠陀植物锯叶乳香可能对减轻炎症性肠病患者的腹泻有效。在本研究中，我们评估了锯叶乳香树胶树脂提取物（BSE）对啮齿动物肠道运动和腹泻的影响。BSE抑制了离体豚鼠回肠由电、乙酰胆碱和氯化钡引起的收缩，对抑制乙酰胆碱和氯化钡引起的收缩更有效。L型钙通道阻滞剂维拉帕米和硝苯地平可降低BSE对乙酰胆碱诱导收缩的抑制作用，但肌浆网钙ATP酶抑制剂环匹阿尼酸、磷酸二酯酶IV抑制剂咯利普兰或脂氧合酶抑制剂齐留通则不能。锯叶乳香的主要活性成分之一3-乙酰-11-酮-β-乳香酸抑制了乙酰胆碱诱导的收缩。BSE抑制了巴豆油处理小鼠的上消化道转运以及蓖麻油诱导的腹泻。然而，BSE对对照小鼠的小肠和大肠的肠道运动均无影响。得出的结论是，BSE通过涉及L型钙通道的机制直接抑制肠道运动。BSE可预防腹泻并使病理生理状态下的肠道运动正常化，而不会减慢对照动物的转运速度。这些结果至少可以部分解释这种阿育吠陀药物在减轻炎症性肠病患者腹泻方面的临床疗效。
