Esfandiari N, Kohi Habibi M, Mosahebi G H, Mozafari J
Department of Plant Protection, Faculty of Agriculture Tehran University, Karaj, Iran.
Commun Agric Appl Biol Sci. 2005;70(3):411-6.
An intensive survey was conducted to identify virus diseases affecting pea crops in Tehran province of Iran. A total of 270 pea samples were collected randomly from pea fields. samples were tested by Double Antibody Sandwich Enzyme Linked Immunosorbent Assay (DAS-ELISA) using polyclonal antisera prepared against PSBMV (AS-0129, DSMZ, Braunschweig, Germany) and TSWV (AS-0580, DSMZ, Braunschweig, Germany). Virus disease incidence in pea samples was followed by PSBMV (33%) TSWV (24.4%) and PSBMV+TSWV (17.77). The positive samples with PSBMV were extracted in 0.05M phosphate buffer pH 6.5-7 containing 2% pvp and inoculated on Pisum sativum, Vicia faba, Chenopodium quinoa, Chenopodium amaranticolor. That produced in Pisum sativum; leaflets roll downwards, shoots curl, internodes shorten and plants are rosetted. Early infections reduce flower and fruit formation or eliminate their development. Broad bean has symptoms accompanied by a certain margin rolling and leaflet distortion. In Chenopodium amaranticolor necrotic local lesions and Chenopodium quinoa chlorotic local lesions had produced. The positive samples with TSWV were extracted in 0.01 M phosphate buffer containing 1% Na2 SO3 and inoculated on Petunia hybrida, Pisum sativum. TSWV causes several symptoms in infected peas, including brown leaf petiole and stem coloration, leaflet spotting, vein necrosis. In petunia hybrida after approximately 5 days showed local necrotic lesion. Biological purification in TSWV with chlorotic local lesions in Petunia hybrida and in PSBMV; chlorotic local lesions in Chenopodium quinoa were done. In PSBMV, back inoculated on Pisum sativum and Vicia faba also tested with DAS-ELISA. RT-PCR confirmed the results. This is the first report of PSBMV and TSWV naturally infecting pea in Iran.
在伊朗德黑兰省开展了一项深入调查，以确定影响豌豆作物的病毒病。从豌豆田随机采集了总共270份豌豆样本。使用针对豌豆南方菜豆花叶病毒（PSBMV，AS - 0129，德国不伦瑞克DSMZ）和番茄斑萎病毒（TSWV，AS - 0580，德国不伦瑞克DSMZ）制备的多克隆抗血清，通过双抗体夹心酶联免疫吸附测定（DAS - ELISA）对样本进行检测。豌豆样本中病毒病的发病率为：PSBMV（33%）、TSWV（24.4%）以及PSBMV + TSWV（17.77%）。带有PSBMV的阳性样本在含2%聚乙烯吡咯烷酮（PVP）的pH 6.5 - 7的0.05M磷酸盐缓冲液中提取，并接种到豌豆、蚕豆、藜麦、千日红上。在豌豆上产生的症状为：小叶向下卷曲、嫩枝卷曲、节间缩短且植株呈莲座状。早期感染会减少花和果实的形成或使其发育受阻。蚕豆出现的症状伴有一定程度的叶缘卷曲和小叶畸变。在千日红上产生坏死局部病斑，在藜麦上产生褪绿局部病斑。带有TSWV的阳性样本在含1%亚硫酸钠的0.01M磷酸盐缓冲液中提取，并接种到矮牵牛、豌豆上。TSWV在受感染的豌豆中会引发多种症状，包括褐色叶柄和茎变色、小叶出现斑点、叶脉坏死。在矮牵牛上接种约5天后出现局部坏死病斑。利用矮牵牛上的褪绿局部病斑对TSWV进行生物学纯化，利用藜麦上的褪绿局部病斑对PSBMV进行生物学纯化。对于PSBMV，回接到豌豆和蚕豆上后也用DAS - ELISA进行检测。逆转录聚合酶链反应（RT - PCR）证实了结果。这是PSBMV和TSWV在伊朗自然感染豌豆的首次报道。
