Hong Baoyu, Haddad Majd, Maley Frank, Jensen Jan H, Kohen Amnon
Department of Chemistry, University of Iowa, Iowa City, Iowa 52242-1294, USA.
J Am Chem Soc. 2006 May 3;128(17):5636-7. doi: 10.1021/ja060196o.
The nature of a H-transfer in the thymidylate synthase catalyzed reaction was investigated by comparison of the wild-type enzyme with the W80M mutant. The nature of the H-transfer was not affected, as indicated by intrinsic isotope effects and their temperature dependence. These findings support a single-step hydride transfer instead of a two-step radical transfer.
通过将野生型酶与W80M突变体进行比较，研究了胸苷酸合成酶催化反应中氢转移的性质。如内在同位素效应及其温度依赖性所示，氢转移的性质未受影响。这些发现支持单步氢化物转移而非两步自由基转移。
