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靶向严重急性呼吸综合征相关冠状病毒结构基因和复制酶基因的小干扰RNA的动力学及协同效应

Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus.

作者信息

He Ming-Liang, Zheng Bo-jian, Chen Ying, Wong Kin-Ling, Huang Jian-Dong, Lin Marie C, Peng Ying, Yuen Kwok Y, Sung Joseph J Y, Kung Hsiang-fu

机构信息

The Center for Emerging Infectious, Li Ka-Shing Medical Institute, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

出版信息

FEBS Lett. 2006 May 1;580(10):2414-20. doi: 10.1016/j.febslet.2006.03.066. Epub 2006 Mar 30.

DOI:10.1016/j.febslet.2006.03.066
PMID:16638566
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7094648/
Abstract

SARS-associated coronavirus was identified as the etiological agent of severe acute respiratory syndrome and a large virus pool was identified in wild animals. Virus generates drug resistance through fast mutagenesis and escapes antiviral treatment. siRNAs targeting different genes would be an alternative for overcoming drug resistance. Here, we report effective siRNAs targeting structural genes (i.e., spike, envelope, membrane, and nucleocapsid) and their antiviral kinetics. We also showed the synergistic effects of two siRNAs targeting different functional genes at a very low dose. Our findings may pave a way to develop cost effective siRNA agents for antiviral therapy in the future.

摘要

严重急性呼吸综合征相关冠状病毒被确定为严重急性呼吸综合征的病原体，并且在野生动物中发现了一个庞大的病毒库。病毒通过快速突变产生耐药性并逃避抗病毒治疗。靶向不同基因的小干扰RNA（siRNA）将是克服耐药性的一种替代方法。在此，我们报告了靶向结构基因（即刺突蛋白、包膜蛋白、膜蛋白和核衣壳蛋白）的有效siRNA及其抗病毒动力学。我们还展示了两种靶向不同功能基因的siRNA在极低剂量下的协同作用。我们的研究结果可能为未来开发具有成本效益的siRNA抗病毒治疗药物铺平道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/d8d3cffd5b93/FEB2-580-2414-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/e353191b46b9/FEB2-580-2414-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/49bd04e43d5f/FEB2-580-2414-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/98e92ca9ba4a/FEB2-580-2414-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/cf8cfde97266/FEB2-580-2414-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/a27531d5ae75/FEB2-580-2414-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/d8d3cffd5b93/FEB2-580-2414-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/e353191b46b9/FEB2-580-2414-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/49bd04e43d5f/FEB2-580-2414-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/98e92ca9ba4a/FEB2-580-2414-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/cf8cfde97266/FEB2-580-2414-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/a27531d5ae75/FEB2-580-2414-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9276/7159376/d8d3cffd5b93/FEB2-580-2414-g006.jpg

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