一种从小鼠建立的肾小管细胞系，该小鼠携带温度敏感型猿猴病毒40大T抗原基因。
A tubule cell line established from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.
作者信息
Yanai N, Satoh T, Kyo S, Abe K, Suzuki M, Obinata M
机构信息
Department of Cell Biology, Research Institute for Tuberculosis and Cancer, Sendai.
出版信息
Jpn J Cancer Res. 1991 Dec;82(12):1344-8. doi: 10.1111/j.1349-7006.1991.tb01803.x.
Abstract
Immortalization of cell lines with specific functions is important for examination of organ-specific functions in vitro. We established a kidney tubule cell line (TKC2) exhibiting a specific physiological response to hormone from the primary culture of kidneys of adult transgenic mice harboring temperature-sensitive SV40 large T-antigen gene. TKC2 cells showed temperature-sensitive growth in culture and exhibited characters of distal tubule cells such as dome formation at confluent culture and stimulation of cAMP synthesis by arginine vasopressin. These phenotypes are maintained after long passages in culture and may provide a new experimental system for studying renal physiology.
摘要
具有特定功能的细胞系永生化对于体外研究器官特异性功能很重要。我们从携带温度敏感型SV40大T抗原基因的成年转基因小鼠肾脏原代培养物中建立了一种肾小管细胞系（TKC2），该细胞系对激素表现出特定的生理反应。TKC2细胞在培养中表现出温度敏感型生长，并表现出远端小管细胞的特征，如汇合培养时形成穹顶以及精氨酸加压素刺激cAMP合成。这些表型在长期传代培养后得以维持，可能为研究肾脏生理学提供一个新的实验系统。
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