Faculty of Dentistry, Tanta University, El-Giesh St., Tanta, Gharbia, Egypt.
Sci Rep. 2024 Nov 27;14(1):29518. doi: 10.1038/s41598-024-80601-0.
This study aimed to evaluate and compare the effectiveness of mineral trioxide aggregate (MTA), bioceramic putty (BP) and tannic acid (TA) for experimental pulpotomy. Our in-vivo experimental study involved sample of 45 rats that were randomly divided into 4 groups: Group 1 (subdivided into negative (1-A) and positive (1-B) subgroups), Group 2 (MTA treated), Group 3 (BP treated) and Group 4 (TA treated). 4 weeks post pulpotomy, specimens were analyzed histologically, immunohistochemically using dentin sialoprotein marker, and histomorphometrically by assessing the thickness of newly formed dentin bridge. Group 1-B showed pulp necrosis without hard tissue formation. Group 2 showed moderate dentin formation while group 3 presented a thick layer of calcific barrier. Group 4 showed dentin bridge formation, however, irregular pulp calcifications and radicular pulp necrosis were seen. The thickness of newly formed dentin bridge showed a significant difference between group 1-B and group 2, 3 &4. Significant difference was found between group 2&3 and group 3&4. Dentin sialoprotein immunohistochemical expression was negative in group 1-B, mild in group 2, strong in group 3 and moderate in group 4. MTA and BP proved to be effective pulpotomy agents with BP being superior. For TA, further studies are required to explain the recorded unfavorable effects in some specimens.
本研究旨在评估和比较三氧化矿物聚合体（MTA）、生物陶瓷腻子（BP）和鞣酸（TA）在实验性活髓切断术中的效果。我们的体内实验研究涉及 45 只大鼠的样本，这些大鼠被随机分为 4 组：第 1 组（分为阴性组 1-A 和阳性组 1-B）、第 2 组（MTA 处理组）、第 3 组（BP 处理组）和第 4 组（TA 处理组）。活髓切断术后 4 周，通过组织学、牙本质涎蛋白标记物的免疫组织化学和新形成牙本质桥的组织形态计量学评估来分析标本。1-B 组表现为牙髓坏死，无硬组织形成。2 组显示出中等程度的牙本质形成，而 3 组呈现出厚厚的钙化屏障层。4 组显示出牙本质桥形成，但也观察到不规则的牙髓钙化和根髓坏死。新形成牙本质桥的厚度在 1-B 组和 2、3 和 4 组之间有显著差异。2、3 组和 3、4 组之间也存在显著差异。1-B 组牙本质涎蛋白免疫组织化学表达阴性，2 组弱阳性，3 组强阳性，4 组中度阳性。MTA 和 BP 被证明是有效的活髓切断剂，BP 更具优势。对于 TA，需要进一步的研究来解释一些标本中记录到的不利影响。
