通过自动套式聚合酶链反应检测食管鳞状细胞癌中的人乳头瘤病毒DNA

Detection of human papillomavirus DNA in squamous cell carcinoma of the esophagus by auto-nested PCR.

作者信息

Souto Damin A P, Guedes Frazzon A P, de Carvalho Damin D, Beck Biehl H, Abruzzi de Oliveira L, Auler R, Marroni C, Alexandre C O P

机构信息

Post-Graduation Program in Pathology, Fundacao Faculdade Federal de Ciencias Medicas de Porto Alegre, Porto Alegre, Brazil.

出版信息

Dis Esophagus. 2006;19(2):64-8. doi: 10.1111/j.1442-2050.2006.00541.x.

DOI:10.1111/j.1442-2050.2006.00541.x

PMID:16643171

Abstract

The aim of the present study was to investigate the presence of human papillomavirus (HPV) in surgical specimens of esophageal squamous cell carcinoma. One hundred and sixty-five paraffin-embedded specimens of esophageal carcinoma were analyzed through high-sensitivity auto-nested polymerase chain reaction (PCR) using the consensus GP5+/GP6+ primer. Twenty-six specimens of esophageal mucosa without malignant disease were also studied as a control group. Two different specific primer sets targeting the E6 region of the HPVs 16 and 18 were used for typing. Direct DNA sequence analysis was conducted to confirm positive PCR results. HPV DNA was detected in 26 esophageal carcinomas (15.75%), but in none of the benign esophageal specimens (P < 0.05). Out of the 26 positive cases, 24 were HPV-16 and one was HPV-18. One tumor contained both HPV-16 and -18 DNA. Positive PCR results were confirmed by the amplified viral sequences. Our findings suggest that the presence of either HPV-16 or -18 might be related to development of the malignant phenotype in the esophagus.

摘要

本研究的目的是调查食管鳞状细胞癌手术标本中人类乳头瘤病毒（HPV）的存在情况。使用通用引物GP5+/GP6+，通过高灵敏度自动巢式聚合酶链反应（PCR）对165例食管癌石蜡包埋标本进行分析。另外选取26例无恶性病变的食管黏膜标本作为对照组进行研究。使用两组不同的针对HPV 16和18型E6区域的特异性引物进行分型。通过直接DNA序列分析来确认PCR阳性结果。在26例食管癌标本中检测到HPV DNA（15.75%），而在所有良性食管标本中均未检测到（P < 0.05）。在26例阳性病例中，24例为HPV - 16型，1例为HPV - 18型。1例肿瘤同时含有HPV - 16和 - 18型DNA。PCR阳性结果通过扩增的病毒序列得到确认。我们的研究结果表明，HPV - 16或 - 18型的存在可能与食管恶性表型的发生有关。