Shitandi Anakalo, Oketch Aila, Mahungu Symon
Guidford Institute, Microbiology Laboratory, Egerton University Njoro, Njoro 20107, Kenya.
J Vet Sci. 2006 Jun;7(2):177-80. doi: 10.4142/jvs.2006.7.2.177.
A Bacillus stearothermophilus var. calidolactis C953 tube test was evaluated for its ability in detecting the residue of selected anticoccidial drugs in poultry, specially sulfamethazine, furazolidone, and amprolium. Various concentrations of each drug were injected into chicken liver and kidney tissues and these tissues were tested to determine the drug detection limits for each drug. The detection limit was defined as the drug concentration at which 95 % of the test results were interpreted as positive. The limits of detection in liver tissue were 0.35 microgram/ml for furazolidone, 0.70 microgram/ml for sulfamethazine and 7.80 microgram/ml for amprolium. In kidney tissues, they were 0.30 microgram/ml for furazolidone, 0.54 microgram/ml for sulfamethazine, and 7.6 microgram/ml for amprolium. It was concluded that this tube test could be used to screen for the residue of these three drugs in poultry.
对嗜热脂肪芽孢杆菌嗜热栖热亚种C953管试法检测家禽中所选抗球虫药残留的能力进行了评估，特别是磺胺二甲嘧啶、呋喃唑酮和氨丙啉。将每种药物的不同浓度注入鸡的肝脏和肾脏组织，并对这些组织进行检测以确定每种药物的检测限。检测限定义为95%的检测结果被判定为阳性时的药物浓度。肝脏组织中呋喃唑酮的检测限为0.35微克/毫升，磺胺二甲嘧啶为0.70微克/毫升，氨丙啉为7.80微克/毫升。在肾脏组织中，呋喃唑酮为0.30微克/毫升，磺胺二甲嘧啶为0.54微克/毫升，氨丙啉为7.6微克/毫升。得出的结论是，这种管试法可用于筛查家禽中这三种药物的残留。
