• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

糊粉层表达基因在萌发的L.种子淀粉胚乳细胞壁甘露聚糖水解中的可能作用。

A Possible Role of the Aleurone Expressed Gene in the Hydrolysis of the Cell Wall Mannans of the Starchy Endosperm in Germinating L. Seeds.

作者信息

Iglesias-Fernández Raquel, Pastor-Mora Elena, Vicente-Carbajosa Jesús, Carbonero Pilar

机构信息

Centro de Biotecnología y Genómica de Plantas-Severo Ochoa (CBGP, UPM-INIA), Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Pozuelo de Alarcón, Spain.

Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Madrid, Spain.

出版信息

Front Plant Sci. 2020 Jan 20;10:1706. doi: 10.3389/fpls.2019.01706. eCollection 2019.

DOI:10.3389/fpls.2019.01706
PMID:32038680
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6983769/
Abstract

The barley endo-β-mannanase () gene family () has been identified and the expression of its members analyzed throughout different plant organs, and upon grain development and germination. The gene has been found to be highly expressed in developing and germinating grains. The MAN (EC 3.2.1.78) enzymatic activity gets a maximum in grains at 48 h of germination (post-germination event). Immunolocalization of mannan polymers in grains has revealed the presence of these polysaccharides in the endosperm cell walls (CWs). By mRNA hybridization assays, the transcripts have been localized to the aleurone layer, but not to the dead starchy endosperm cells. These data suggest that MAN1 is synthesized in the aleurone layer during early grain imbibition and moves potentially through the apoplast to the endosperm where the hydrolysis of the mannan polymers takes place after germination . Hence, mannans in the starchy endosperm CWs, besides their structural function, could be used as reserve compounds upon barley post-germination.

摘要

大麦内切-β-甘露聚糖酶（）基因家族（）已被鉴定，并且其成员在不同植物器官以及籽粒发育和萌发过程中的表达也已得到分析。已发现基因在发育中和萌发的籽粒中高度表达。MAN（EC 3.2.1.78）酶活性在萌发48小时的籽粒中达到最大值（萌发后事件）。籽粒中甘露聚糖聚合物的免疫定位揭示了这些多糖存在于胚乳细胞壁（CWs）中。通过mRNA杂交试验，转录本已定位到糊粉层，而未定位到死亡的淀粉胚乳细胞。这些数据表明，MAN1在籽粒早期吸水过程中在糊粉层中合成，并可能通过质外体移动到胚乳，在萌发后甘露聚糖聚合物在胚乳中发生水解。因此，淀粉胚乳CWs中的甘露聚糖，除了其结构功能外，在大麦萌发后还可作为储备化合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/5463a7d31761/fpls-10-01706-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/4588c641a840/fpls-10-01706-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/fcfcc7f663a8/fpls-10-01706-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/221ded5307e0/fpls-10-01706-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/95d88feea009/fpls-10-01706-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/497ff33db2ba/fpls-10-01706-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/9d0d5600febb/fpls-10-01706-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/5463a7d31761/fpls-10-01706-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/4588c641a840/fpls-10-01706-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/fcfcc7f663a8/fpls-10-01706-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/221ded5307e0/fpls-10-01706-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/95d88feea009/fpls-10-01706-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/497ff33db2ba/fpls-10-01706-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/9d0d5600febb/fpls-10-01706-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8b7/6983769/5463a7d31761/fpls-10-01706-g007.jpg

相似文献

1
A Possible Role of the Aleurone Expressed Gene in the Hydrolysis of the Cell Wall Mannans of the Starchy Endosperm in Germinating L. Seeds.糊粉层表达基因在萌发的L.种子淀粉胚乳细胞壁甘露聚糖水解中的可能作用。
Front Plant Sci. 2020 Jan 20;10:1706. doi: 10.3389/fpls.2019.01706. eCollection 2019.
2
Mannans and endo-β-mannanases (MAN) in Brachypodium distachyon: expression profiling and possible role of the BdMAN genes during coleorhiza-limited seed germination.短柄草中的甘露聚糖和内切-β-甘露聚糖酶（MAN）：BdMAN基因在胚根鞘限制型种子萌发过程中的表达谱分析及可能作用
J Exp Bot. 2015 Jul;66(13):3753-64. doi: 10.1093/jxb/erv168. Epub 2015 Apr 28.
3
Hydrolysis of (1,4)-beta-D-mannans in barley (Hordeum vulgare L.) is mediated by the concerted action of (1,4)-beta-D-mannan endohydrolase and beta-D-mannosidase.大麦（Hordeum vulgare L.）中（1,4）-β-D-甘露聚糖的水解是由（1,4）-β-D-甘露聚糖内切水解酶和β-D-甘露糖苷酶的协同作用介导的。
Biochem J. 2006 Oct 1;399(1):77-90. doi: 10.1042/BJ20060170.
4
Three endo-β-mannanase genes expressed in the micropylar endosperm and in the radicle influence germination of Arabidopsis thaliana seeds.三个在内胚乳和胚根中表达的内-β-甘露聚糖酶基因影响拟南芥种子的萌发。
Planta. 2011 Jan;233(1):25-36. doi: 10.1007/s00425-010-1257-z. Epub 2010 Sep 28.
5
Mannans and endo-β-mannanase transcripts are located in different seed compartments during Brassicaceae germination.甘露聚糖和内切-β-甘露聚糖酶的转录本在十字花科种子萌发过程中位于不同的种子隔室中。
Planta. 2018 Mar;247(3):649-661. doi: 10.1007/s00425-017-2815-4. Epub 2017 Nov 21.
6
Differences in hydrolytic enzyme activity accompany natural variation in mature aleurone morphology in barley (Hordeum vulgare L.).在大麦（Hordeum vulgare L.）中，成熟糊粉层形态的自然变异伴随着水解酶活性的差异。
Sci Rep. 2018 Jul 23;8(1):11025. doi: 10.1038/s41598-018-29068-4.
7
Localization of carboxypeptidase I in germinating barley grain.羧肽酶 I 在发芽大麦粒中的定位。
Plant Physiol. 1990 Aug;93(4):1449-52. doi: 10.1104/pp.93.4.1449.
8
Secretion of alpha-amylase by the aleurone layer and the scutellum of germinating barley grain.萌发大麦粒的糊粉层和盾片对α-淀粉酶的分泌。
Plant Physiol. 1984 Jul;75(3):710-5. doi: 10.1104/pp.75.3.710.
9
Softening-up mannan-rich cell walls.软化富含甘露聚糖的细胞壁。
J Exp Bot. 2012 Jun;63(11):3976-88. doi: 10.1093/jxb/ers096. Epub 2012 May 2.
10
Isolation of tissues and preservation of RNA from intact, germinated barley grain.从完整的、发芽的大麦粒中分离组织并保存 RNA。
Plant J. 2017 Aug;91(4):754-765. doi: 10.1111/tpj.13600. Epub 2017 Jun 15.

引用本文的文献

1
SCL15 Regulates the Release of Seed Dormancy in Arabidopsis thaliana by Integrating the Circadian Clock, Hormonal Signals and Cell Wall Remodelling.SCL15通过整合生物钟、激素信号和细胞壁重塑来调控拟南芥种子休眠的解除。
Physiol Plant. 2025 Sep-Oct;177(5):e70467. doi: 10.1111/ppl.70467.
2
Genome-wide systematic survey and analysis of the RNA helicase gene family and their response to abiotic stress in sweetpotato.甘薯中 RNA 解旋酶基因家族的全基因组系统调查和分析及其对非生物胁迫的响应。
BMC Plant Biol. 2024 Mar 16;24(1):193. doi: 10.1186/s12870-024-04824-z.
3
Variety-dependent accumulation of glucomannan in the starchy endosperm and aleurone cell walls of rice grains and its possible genetic basis.

本文引用的文献

1
Fenugreek seed gum: Biological properties, chemical modifications, and structural analysis- A review.葫芦巴胶：生物特性、化学修饰及结构分析——综述。
Int J Biol Macromol. 2019 Oct 1;138:386-393. doi: 10.1016/j.ijbiomac.2019.07.006. Epub 2019 Jul 2.
2
Insights on the Proteases Involved in Barley and Wheat Grain Germination.大麦和小麦发芽过程中涉及的蛋白酶研究进展。
Int J Mol Sci. 2019 Apr 28;20(9):2087. doi: 10.3390/ijms20092087.
3
Editorial: Seed Dormancy, Germination, and Pre-harvest Sprouting.社论：种子休眠、萌发与收获前发芽
水稻籽粒淀粉胚乳和糊粉层细胞壁中葡甘露聚糖的品种依赖性积累及其可能的遗传基础。
Plant Biotechnol (Tokyo). 2023 Dec 25;40(4):321-336. doi: 10.5511/plantbiotechnology.23.0809a.
4
Identification of Reference Genes for Precise Expression Analysis during Germination in Seeds under Salt Stress.盐胁迫下种子萌发过程中精确表达分析的参考基因鉴定。
Int J Mol Sci. 2023 Nov 1;24(21):15878. doi: 10.3390/ijms242115878.
5
Roles of Reactive Oxygen Species and Mitochondria in Seed Germination.活性氧物种和线粒体在种子萌发中的作用。
Front Plant Sci. 2021 Dec 9;12:781734. doi: 10.3389/fpls.2021.781734. eCollection 2021.
6
Tissue-Specific Proteome and Subcellular Microscopic Analyses Reveal the Effect of High Salt Concentration on Actin Cytoskeleton and Vacuolization in Aleurone Cells during Early Germination of Barley.组织特异性蛋白质组学和亚细胞显微镜分析揭示了高盐浓度对大麦早期萌发过程中糊粉层细胞肌动蛋白细胞骨架和液泡化的影响。
Int J Mol Sci. 2021 Sep 6;22(17):9642. doi: 10.3390/ijms22179642.
7
Delayed hydrolysis of Raffinose Family Oligosaccharides (RFO) affects critical germination of chickpeas.棉子糖家族寡糖（RFO）的延迟水解会影响鹰嘴豆的关键萌发过程。
3 Biotech. 2021 Jun;11(6):298. doi: 10.1007/s13205-021-02764-1. Epub 2021 May 26.
Front Plant Sci. 2018 Nov 30;9:1783. doi: 10.3389/fpls.2018.01783. eCollection 2018.
4
Mannans and endo-β-mannanase transcripts are located in different seed compartments during Brassicaceae germination.甘露聚糖和内切-β-甘露聚糖酶的转录本在十字花科种子萌发过程中位于不同的种子隔室中。
Planta. 2018 Mar;247(3):649-661. doi: 10.1007/s00425-017-2815-4. Epub 2017 Nov 21.
5
Carbon Supply and the Regulation of Cell Wall Synthesis.碳供应与细胞壁合成的调控。
Mol Plant. 2018 Jan 8;11(1):75-94. doi: 10.1016/j.molp.2017.10.004. Epub 2017 Oct 17.
6
An Overview of Protein Secretion in Plant Cells.植物细胞中蛋白质分泌的概述。
Methods Mol Biol. 2017;1662:19-32. doi: 10.1007/978-1-4939-7262-3_2.
7
The biomechanics of seed germination.种子萌发的生物力学
J Exp Bot. 2017 Feb 1;68(4):765-783. doi: 10.1093/jxb/erw428.
8
Cell wall degradation is required for normal starch mobilisation in barley endosperm.细胞壁降解是大麦胚乳中正常淀粉动员所必需的。
Sci Rep. 2016 Sep 13;6:33215. doi: 10.1038/srep33215.
9
Mannans and endo-β-mannanases (MAN) in Brachypodium distachyon: expression profiling and possible role of the BdMAN genes during coleorhiza-limited seed germination.短柄草中的甘露聚糖和内切-β-甘露聚糖酶（MAN）：BdMAN基因在胚根鞘限制型种子萌发过程中的表达谱分析及可能作用
J Exp Bot. 2015 Jul;66(13):3753-64. doi: 10.1093/jxb/erv168. Epub 2015 Apr 28.
10
Programmed cell death (PCD): an essential process of cereal seed development and germination.程序性细胞死亡（PCD）：谷物种子发育和萌发的一个必要过程。
Front Plant Sci. 2014 Jul 28;5:366. doi: 10.3389/fpls.2014.00366. eCollection 2014.