Seiler N, Knödgen B
J Chromatogr. 1978 Jan 1;145(1):29-39. doi: 10.1016/s0378-4347(00)81665-5.
Using a Lichrosorb RP-8 reversed-phase column and a methanol--water gradient elution program, it is possible to separate within 40 min and to determine routinely in picomole quantities the natural di- and polyamines. The precision of the method is comparable to the thin-layer chromatographic procedures, the separations are most efficient, and the method can be fully automated. A modified gradient enables the repeated assay of spermidine and spermine within 20 min. The method is suited for polyamine analyses in tissues and body fluids.
使用Lichrosorb RP - 8反相柱和甲醇 - 水梯度洗脱程序，能够在40分钟内分离并常规测定皮摩尔量的天然二胺和多胺。该方法的精密度与薄层色谱法相当，分离效率最高，且该方法可完全自动化。一种改进的梯度能够在20分钟内重复测定亚精胺和精胺。该方法适用于组织和体液中的多胺分析。
