大豆种子中的玻璃化转变：与无水生物学的相关性。
Glass transitions in soybean seed : relevance to anhydrous biology.
机构信息
Section of Plant Biology, Cornell University, Ithaca, New York 14853.
出版信息
Plant Physiol. 1991 Jun;96(2):660-3. doi: 10.1104/pp.96.2.660.
Abstract
We have investigated the mechanism by which anhydrobiotic organisms can survive severe dehydration. The method used was measurement of the rotational diffusion coefficient of a hydrophilic spin probe, inserted in the cytoplasm of soybean (Glycine max L.) axes, as a function of temperature and sample water content. Results indicate the existence of a hydration-dependent glass-like transition at physiological temperatures. No glass transitions have been observed in desiccation-intolerant samples, suggesting that the ability to withstand dehydration is associated with glass formation.
摘要
我们研究了耐缺水生物在严重脱水条件下存活的机制。使用的方法是测量亲水性旋转探针的旋转扩散系数，该探针插入大豆（Glycine max L.）轴的细胞质中，作为温度和样品含水量的函数。结果表明，在生理温度下存在依赖于水合作用的玻璃化转变。在不耐干燥的样品中没有观察到玻璃化转变，这表明耐受干燥的能力与玻璃形成有关。
相似文献
1
Glass transitions in soybean seed : relevance to anhydrous biology.大豆种子中的玻璃化转变：与无水生物学的相关性。
Plant Physiol. 1991 Jun;96(2):660-3. doi: 10.1104/pp.96.2.660.
2
Pools of water in anhydrobiotic organisms: A thermally stimulated depolarization current study.脱水生物体内的水潭：热刺激去极化电流研究。
Biophys J. 1992 Sep;63(3):663-72. doi: 10.1016/S0006-3495(92)81638-7.
3
Calorimetric Properties of Dehydrating Pollen (Analysis of a Desiccation-Tolerant and an Intolerant Species).脱水花粉的量热特性（对一种耐干燥和一种不耐干燥物种的分析）
Plant Physiol. 1996 May;111(1):235-242. doi: 10.1104/pp.111.1.235.
4
Quantification of Protein Hydration, Glass Transitions, and Structural Relaxations of Aqueous Protein and Carbohydrate-Protein Systems.蛋白质水合作用、玻璃化转变以及蛋白质水溶液和碳水化合物-蛋白质体系的结构弛豫的量化
J Phys Chem B. 2015 Jun 11;119(23):7077-86. doi: 10.1021/acs.jpcb.5b01593. Epub 2015 May 27.
5
High-temperature tolerance in anhydrobiotic tardigrades is limited by glass transition.水生熊虫在脱水状态下的高温耐受性受玻璃化转变限制。
Physiol Biochem Zool. 2009 Nov-Dec;82(6):749-55. doi: 10.1086/605954.
6
Thermal dehydration of magnesium acetate tetrahydrate: formation and in situ crystallization of anhydrous glass.四水醋酸镁的热脱水：无水玻璃的形成和原位结晶。
J Phys Chem B. 2012 Dec 13;116(49):14477-86. doi: 10.1021/jp3052517. Epub 2012 Dec 4.
7
The anhydrobiotic potential and molecular phylogenetics of species and strains of Panagrolaimus (Nematoda, Panagrolaimidae).类小杆线虫属（线虫纲，类小杆线虫科）物种及菌株的隐生潜能与分子系统发育学
J Exp Biol. 2005 Jun;208(Pt 12):2433-45. doi: 10.1242/jeb.01629.
8
Effects of dehydration temperatures on moisture absorption and dissolution behavior of theophylline.脱水温度对茶碱吸湿及溶解行为的影响
Chem Pharm Bull (Tokyo). 2001 Dec;49(12):1526-30. doi: 10.1248/cpb.49.1526.
9
Desiccation-induced structuralization and glass formation of group 3 late embryogenesis abundant protein model peptides.干燥诱导的第 3 组晚期胚胎丰富蛋白模型肽的结构化和玻璃化。
Biochemistry. 2010 Feb 16;49(6):1093-104. doi: 10.1021/bi901745f.
10
Sugars and desiccation tolerance in seeds.种子中的糖类与脱水耐受性
Plant Physiol. 1988 Nov;88(3):829-32. doi: 10.1104/pp.88.3.829.
引用本文的文献
1
Localization of Lipid Droplets in Embryonic Axis Radicle Cells of Soybean Seeds under Various Imbibition Regimes Indicates Their Role in Desiccation Tolerance.不同吸胀条件下大豆种子胚轴胚根细胞中脂滴的定位表明其在耐干性中的作用。
Plants (Basel). 2023 Feb 10;12(4):799. doi: 10.3390/plants12040799.
2
Mechanisms of Desiccation Tolerance: Themes and Variations in Brine Shrimp, Roundworms, and Tardigrades.耐干燥机制：卤虫、蛔虫和缓步动物的主题与变化
Front Physiol. 2020 Oct 23;11:592016. doi: 10.3389/fphys.2020.592016. eCollection 2020.
3
The biology of tardigrade disordered proteins in extreme stress tolerance.水熊虫抗极端压力紊乱蛋白的生物学特性。
Cell Commun Signal. 2020 Nov 4;18(1):178. doi: 10.1186/s12964-020-00670-2.
4
Synergistic Development of Biochips and Cell Preservation Methodologies: A Tale of Converging Technologies.生物芯片与细胞保存方法的协同发展：融合技术的故事
Curr Stem Cell Rep. 2017 Mar;3(1):45-53. doi: 10.1007/s40778-017-0074-8. Epub 2017 Jan 21.
5
Preservation of differentiation and clonogenic potential of human hematopoietic stem and progenitor cells during lyophilization and ambient storage.在冷冻干燥和常温储存过程中，人类造血干/祖细胞的分化和克隆潜能的保存。
PLoS One. 2010 Sep 1;5(9):e12518. doi: 10.1371/journal.pone.0012518.
6
Development of desiccation tolerance and vitrification by preculture treatment in suspension-cultured cells of the liverwort Marchantia polymorpha.通过悬浮培养的地钱属（Marchantia polymorpha）细胞的预培养处理来开发干燥耐受性和玻璃化。
Planta. 2010 Mar;231(4):965-76. doi: 10.1007/s00425-010-1101-5. Epub 2010 Jan 26.
7
Pools of water in anhydrobiotic organisms: A thermally stimulated depolarization current study.脱水生物体内的水潭：热刺激去极化电流研究。
Biophys J. 1992 Sep;63(3):663-72. doi: 10.1016/S0006-3495(92)81638-7.
8
Changes in Soluble Carbohydrates during Seed Storage.种子贮藏过程中可溶糖的变化。
Plant Physiol. 1992 Mar;98(3):1207-10. doi: 10.1104/pp.98.3.1207.
9
Maturation proteins and sugars in desiccation tolerance of developing soybean seeds.发育中的大豆种子耐干燥性中的成熟蛋白和糖。
Plant Physiol. 1992 Sep;100(1):225-30. doi: 10.1104/pp.100.1.225.
10
Effect of viscoelastic relaxation on moisture transport in foods. Part II: sorption and drying of soybeans.粘弹性松弛对食品中水分传输的影响。第二部分：大豆的吸附与干燥
J Math Biol. 2004 Jul;49(1):20-34. doi: 10.1007/s00285-003-0250-6. Epub 2004 Jan 2.
本文引用的文献
1
Relationship between Thermal Transitions and Freezing Injury in Pea and Soybean Seeds.豌豆和大豆种子热转变与冻害之间的关系
Plant Physiol. 1989 Jul;90(3):1121-8. doi: 10.1104/pp.90.3.1121.
2
The glassy state in corn embryos.玉米胚的玻璃态。
Plant Physiol. 1989 Mar;89(3):977-81. doi: 10.1104/pp.89.3.977.
3
Sugars and desiccation tolerance in seeds.种子中的糖类与脱水耐受性
Plant Physiol. 1988 Nov;88(3):829-32. doi: 10.1104/pp.88.3.829.
4
The relationship between water binding and desiccation tolerance in tissues.组织中水分结合与耐干燥性之间的关系。
Plant Physiol. 1987;85(1):232-8. doi: 10.1104/pp.85.1.232.
5
Bioenergetic studies of cells with spin probes.
Ann N Y Acad Sci. 1983;414:180-9. doi: 10.1111/j.1749-6632.1983.tb31684.x.
6
Viscosity of cellular protoplasm.细胞原生质的黏度
Science. 1974 Feb 15;183(4125):666-8. doi: 10.1126/science.183.4125.666.
7
Cell water viscosity.
Methods Enzymol. 1986;127:360-9. doi: 10.1016/0076-6879(86)27030-5.
8
Determining intracellular viscosity from the rotational motion of spin labels.通过自旋标记物的旋转运动测定细胞内粘度。
Methods Enzymol. 1986;127:239-49. doi: 10.1016/0076-6879(86)27020-2.
9
Spin label motion in the internal aqueous compartment of spinach thylakoids.
Arch Biochem Biophys. 1979 Apr 15;194(1):138-48. doi: 10.1016/0003-9861(79)90603-9.
Zotero 插件