Mencucci Rita, Menchini Ugo, Dei Rosanna
Department of Oto-Neuro-Opthalmological Surgical Sciences, University of Florence, Florence, Italy.
Cornea. 2006 May;25(4):428-31. doi: 10.1097/01.ico.0000214207.06952.23.
To investigate if amniotic membrane incubated with antibiotics could inhibit bacterial growth in vitro.
Amniotic membrane fragments were incubated with the antibiotics (netilmicin) solution; the washed and drained fragments were either tested after treatment or further incubated in antibiotic-free medium. The antibacterial activity of both amniotic membrane and elution media was carried out by the Agar diffusion method, with Staphylococcus epidermidis as indicator, measuring the inhibition zone after overnight incubation.
The amniotic membrane fragments soaked in antibiotics inhibited bacterial growth. Antibiotic uptake was dose-dependent and occurred rapidly. The drug was released from the membrane, and the antibacterial effect was present in the elution media at least 3 days after treatment.
Our preliminary in vitro data show that amniotic membrane can absorb the antibiotic netilmicin and in the future may be used to deliver antibiotics, as reported for collagen shields and other medical prosthetic devices.
研究与抗生素孵育的羊膜是否能在体外抑制细菌生长。
将羊膜碎片与抗生素（奈替米星）溶液孵育；洗涤并沥干的碎片在处理后进行测试，或在无抗生素培养基中进一步孵育。采用琼脂扩散法以表皮葡萄球菌为指示菌，对羊膜和洗脱介质的抗菌活性进行检测，过夜孵育后测量抑菌圈。
浸泡在抗生素中的羊膜碎片可抑制细菌生长。抗生素摄取呈剂量依赖性且迅速发生。药物从膜中释放，处理后至少3天洗脱介质中仍存在抗菌作用。
我们的初步体外数据表明，羊膜可吸收抗生素奈替米星，未来可能如胶原蛋白护罩和其他医用假体装置一样用于递送抗生素。
