Wang Lianrong, Chen Shi, Xiao Xiang, Huang Xi, You Delin, Zhou Xiufen, Deng Zixin
School of Life Science and Biotechnology, Shanghai Jiaotong University, 1954 Huashan Road, Shanghai 200030, People's Republic of China.
Appl Environ Microbiol. 2006 May;72(5):3738-42. doi: 10.1128/AEM.72.5.3738-3742.2006.
In the arsenic resistance gene cluster from the large linear plasmid pHZ227, two novel genes, arsO (for a putative flavin-binding monooxygenase) and arsT (for a putative thioredoxin reductase), were coactivated and cotranscribed with arsR1-arsB and arsC, respectively. Deletion of the ars gene cluster on pHZ227 in Streptomyces sp. strain FR-008 resulted in sensitivity to arsenic, and heterologous expression of the ars gene cluster in the arsenic-sensitive Streptomyces strains conferred resistance on the new hosts. The pHZ227 ArsB protein showed homology to the yeast arsenite transporter Acr3p. The pHZ227 ArsC appears to be a bacterial thioredoxin-dependent ArsC-type arsenate reductase with four conserved cysteine thioredoxin-requiring motifs.
在来自大型线性质粒pHZ227的抗砷基因簇中，两个新基因arsO（推测为黄素结合单加氧酶）和arsT（推测为硫氧还蛋白还原酶）分别与arsR1 - arsB和arsC共同激活并共转录。链霉菌属菌株FR - 008中pHZ227上ars基因簇的缺失导致对砷敏感，并且ars基因簇在对砷敏感的链霉菌菌株中的异源表达赋予新宿主抗性。pHZ227 ArsB蛋白与酵母亚砷酸盐转运蛋白Acr3p具有同源性。pHZ227 ArsC似乎是一种细菌硫氧还蛋白依赖性的ArsC型砷酸盐还原酶，具有四个保守的需要硫氧还蛋白的半胱氨酸基序。
