Berinstein A, Piatti P, Gaggino O P, Schudel A A, Sadir A M
Institute of Virology, CICV, Moron, Argentina.
Vaccine. 1991 Dec;9(12):883-8. doi: 10.1016/0264-410x(91)90008-t.
The immunomodulating effect of an extract of the cell wall of Mycobacterium sp. (WSF, Vetrepharm Inc., London, Canada) in foot-and-mouth disease virus inactivated vaccines was tested in a murine model. The duration of immunity, protection, stimulation of immunocompetent cells acting on the long-lasting secondary response and possible tissue damage were examined. The incorporation of 10 micrograms WSF into aqueous and oil vaccines induced a high and long-lasting specific antibody response. The neutralizing titres of these antibodies were significantly higher than those observed in animals immunized with vaccines lacking WSF and conferred protection for at least 7 months. The data collected in repopulation assays indicate that WSF participates in the activation of immune cell populations involved in long-lasting memory. This results in an efficient B-cell secondary response even in the absence of T cells, lasting at least 5 months. No adverse reactions were detected. The enhancement effect of WSF on the immune response to foot and mouth disease vaccines observed in the murine model indicates the possibility of its inclusion in aqueous vaccines to be tested in cattle.
在小鼠模型中测试了分枝杆菌属细胞壁提取物（WSF，加拿大伦敦Vetrepharm公司）对口蹄疫病毒灭活疫苗的免疫调节作用。检测了免疫持续时间、保护作用、对作用于持久二次应答的免疫活性细胞的刺激以及可能的组织损伤。将10微克WSF加入水性疫苗和油佐剂疫苗中可诱导产生高且持久的特异性抗体应答。这些抗体的中和效价显著高于用不含WSF的疫苗免疫的动物所观察到的中和效价，并提供至少7个月的保护。在再增殖试验中收集的数据表明，WSF参与了参与持久记忆的免疫细胞群体的激活。即使在没有T细胞的情况下，这也会导致有效的B细胞二次应答，持续至少5个月。未检测到不良反应。在小鼠模型中观察到WSF对口蹄疫疫苗免疫应答的增强作用表明，有可能将其加入待在牛身上进行测试的水性疫苗中。
