Blanchard Sophie, Thorson Jon S
Laboratory for Biosynthetic Chemistry, Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, Wisconsin 53705, USA.
Curr Opin Chem Biol. 2006 Jun;10(3):263-71. doi: 10.1016/j.cbpa.2006.04.001. Epub 2006 May 3.
Glycosylated natural products have served as reliable platforms for the development of many existing front-line drugs. In an effort to explore the contribution of the sugar constituents of these compounds, research groups have focused upon the development of chemical and enzymatic tools to diversify natural product glycosylation. Among the complementary routes available, in vivo pathway engineering, also referred to as 'combinatorial biosynthesis', is an emerging method that relies upon the co-expression of sugar biosynthetic gene cassettes and glycosyltransferases in a host organism to generate novel glycosylated natural products. An overview of recent progress in combinatorial biosynthesis is highlighted in this review, emphasizing the elucidation of nucleotide-sugar biosynthetic pathways and recent developments on glycosyltransferases.
糖基化天然产物一直是许多现有一线药物开发的可靠平台。为了探索这些化合物中糖成分的作用，研究团队致力于开发化学和酶学工具，以实现天然产物糖基化的多样化。在现有的互补途径中，体内途径工程，也称为“组合生物合成”，是一种新兴方法，它依赖于在宿主生物体中共表达糖生物合成基因盒和糖基转移酶，以生成新型糖基化天然产物。本文综述了组合生物合成的最新进展，重点阐述了核苷酸糖生物合成途径的阐明以及糖基转移酶的最新进展。
