Strompfová Viola, Marcináková Miroslava, Simonová Monika, Bogovic-Matijasić Bojana, Lauková Andrea
Institute of Animal Physiology Slovak Academy of Sciences, Soltésovej 4-6, 04001 Kosice, Slovakia.
Anaerobe. 2006 Apr;12(2):75-9. doi: 10.1016/j.anaerobe.2005.12.001. Epub 2006 Feb 15.
Probiotic utilization is becoming increasingly popular in veterinary medicine. However, only few probiotic products are available commercially for use in dogs in our market. Therefore, the aim of our study was to determine the properties of new potential probiotic Lactobacillus fermentum AD1 strain-own canine isolate and to investigate its effect on several microbiological and biochemical parameters in healthy dogs. The strain expressed in vitro survival by pH 3.0 after 3h (86.8%) and in the presence of 1% bile (75.4%). The AD1 strain adhered to the canine and human intestinal mucus. It was sensitive to commonly used antimicrobials. Fifteen healthy dogs were supplemented with 10(9)L. fermentum AD1 for 7 days. At the end of AD1 strain application, numbers of faecal lactobacilli and enterococci increased significantly in the canine faeces. Significant increase of total protein and total lipid and significant reduction of glucose in serum of dogs were noted. These data indicate that L. fermentum AD1 survive transit through the canine gastrointestinal tract, and populate the colon and probably increased absorption of some nutrients. Whether longer time of its application lead to the same results as well as its potential to improve immune function in dogs remains to be determined.
益生菌的应用在兽医学中越来越普遍。然而，在我们的市场上，只有少数益生菌产品可用于商业用途的犬类。因此，我们研究的目的是确定新的潜在益生菌发酵乳杆菌AD1菌株（犬源分离株）的特性，并研究其对健康犬类一些微生物学和生化参数的影响。该菌株在pH 3.0条件下3小时后体外存活率为86.8%，在1%胆汁存在下为75.4%。AD1菌株能黏附于犬类和人类肠道黏液。它对常用抗菌药物敏感。15只健康犬补充10⁹个发酵乳杆菌AD1，持续7天。在应用AD1菌株结束时，犬类粪便中的粪便乳酸杆菌和肠球菌数量显著增加。犬血清中总蛋白和总脂质显著增加，葡萄糖显著降低。这些数据表明，发酵乳杆菌AD1能够在犬类胃肠道中存活并定殖于结肠，可能还增加了某些营养物质的吸收。其更长时间的应用是否会导致相同结果以及对犬类免疫功能的改善潜力仍有待确定。
