Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia.
Probiotics Antimicrob Proteins. 2018 Sep;10(3):577-588. doi: 10.1007/s12602-017-9370-y.
There are growing efforts to find applications for various naturally occurring organo-mineral rocks. They have so far been preferentially used in agriculture and forestry; however, medicine and nutrition may also be interesting areas for their application. This study investigates the effects of dietary supplementation with canine-derived probiotic strain Lactobacillus fermentum CCM 7421 in combination with alginite in dogs. Alginite is a loam-like material of volcanic origin composed of clay minerals and fossilised unicellular algae. The effects of these additives on faecal microbiota, faecal characteristics, short-chain fatty acid profile, haematology, serum biochemistry and cellular immunity parameters were monitored. Forty dogs were randomly divided into four treatment groups: control group (C), alginite-supplemented group (A; 1% diet), probiotic group (LF; L. fermentum CCM 7421 at a dose of 10 cfu/day/dog) and combined group (A + LF group); 10 dogs in each group. The experiment lasted for 35 days with a 14-day treatment period (sample collection at days 0, 7, 14 and 35). The results of this straightforward experiment showed beneficial effects in the combined A + LF group. In detail, a decrease in faecal coliforms and clostridia and an increase in lactic acid bacteria, haemoglobin and serum magnesium levels compared to baseline were observed in the A + LF group (P < 0.05). In contrast, sole application of alginite (A group) led to several unexpected effects such as an increase in clostridial population and serum alanine aminotrasferase and a decrease in haemoglobin concentration (P < 0.05). The addition of alginite prevented a decrease in faecal pH and serum mineral content observed in the LF group. This indicates the possibility of applying alginite also in the nutrition of dogs as a combinative additive with probiotic bacteria for restoring optimal acid-alkali balance without affecting positive probiotic effects.
目前，人们越来越努力地寻找各种天然存在的有机-矿物岩石的应用。迄今为止，它们主要应用于农业和林业；然而，医学和营养也可能是它们应用的有趣领域。本研究调查了犬源益生菌菌株发酵乳杆菌 CCM 7421 与藻土联合在犬中的膳食补充作用。藻土是一种由粘土矿物和化石单细胞藻类组成的火山起源的壤土状物质。监测了这些添加剂对粪便微生物群、粪便特性、短链脂肪酸谱、血液学、血清生化和细胞免疫参数的影响。将 40 只狗随机分为四组：对照组（C）、藻土添加组（A；日粮的 1%）、益生菌组（LF；每天每只狗 10cfu 的发酵乳杆菌 CCM 7421）和联合组（A+LF 组）；每组 10 只狗。实验持续 35 天，治疗期为 14 天（第 0、7、14 和 35 天采集样本）。这项简单的实验结果显示，联合 A+LF 组有有益的效果。具体而言，与基线相比，A+LF 组观察到粪便大肠菌群和梭菌减少，乳酸菌、血红蛋白和血清镁水平增加（P<0.05）。相比之下，单独应用藻土（A 组）导致一些意外的效果，如梭菌种群增加、血清丙氨酸氨基转移酶增加和血红蛋白浓度降低（P<0.05）。藻土的添加阻止了 LF 组粪便 pH 和血清矿物质含量的降低。这表明藻土也有可能作为一种联合添加剂应用于犬的营养中，与益生菌一起用于恢复最佳酸碱平衡，而不影响益生菌的积极作用。
