Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
Microb Pathog. 2019 Jun;131:246-253. doi: 10.1016/j.micpath.2019.04.006. Epub 2019 Apr 8.
Gastrointestinal (GI) infection is one of the most common types of infectious diseases. Application of probiotic strains in the control of such infections represents a promising approach. In this study, Lactobacillus fermentum strain 4-17 was isolated from kashkineh, an Iranian cereal fermented food, and identified by sequencing its 16S rRNA gene using universal primers. Its probiotic features, including resistance to acid, bile tolerance, antibacterial activity, resistance to intestinal and gastric juices, and hydrophobicity were evaluated. The ability of this strain to adhere to human intestinal cells as well as the anti-adhesive effect of L. fermentum strain 4-17 against E. coli isolated from patients with urinary tract infection was investigated. L. fermentum strain 4-17 was capable of surviving at various conditions such as low pH values, bile salts exposure, and GI tract environment. It showed 43% cell hydrophobicity. The adhesion level of L. fermentum strain 4-17 to human colon adenocarcinoma Caco-2 cells was 8.5% which was also confirmed by scanning electron microscopy (SEM). Furthermore, this strain showed appropriate anti-adhesive properties (including competition, inhibition and replacement properties) against human pathogenic bacteria. These data suggest that L. fermentum strain 4-17 could be examined further for its useful effects and introduced as a novel candidate probiotic to control GI infection disease.
胃肠道（GI）感染是最常见的传染病类型之一。应用益生菌菌株来控制此类感染是一种很有前途的方法。在本研究中，从伊朗发酵谷物食品 Kashkineh 中分离出乳杆菌发酵菌株 4-17，并使用通用引物通过测序其 16S rRNA 基因进行鉴定。评估了其益生菌特性，包括耐酸性、胆汁耐受性、抗菌活性、耐肠液和胃液以及疏水性。研究了该菌株对人肠细胞的黏附能力以及 L. fermentum 菌株 4-17 对尿路感染患者分离出的大肠杆菌的抗黏附作用。L. fermentum 菌株 4-17 能够在各种条件下存活，例如低 pH 值、胆汁盐暴露和胃肠道环境。它表现出 43%的细胞疏水性。L. fermentum 菌株 4-17 与人结肠腺癌细胞 Caco-2 的黏附水平为 8.5%，这也通过扫描电子显微镜（SEM）得到了证实。此外，该菌株对人致病菌表现出适当的抗黏附特性（包括竞争、抑制和替代特性）。这些数据表明，L. fermentum 菌株 4-17 可以进一步研究其有益作用，并作为一种新型候选益生菌引入以控制 GI 感染疾病。
