Forsey Richard W, Fisher John, Thompson Jonathan, Stone Martin H, Bell Coral, Ingham Eileen
Department of Chemical Engineering, University of Bath, Bath BA2 7AY, UK.
Biomaterials. 2006 Sep;27(26):4581-90. doi: 10.1016/j.biomaterials.2006.04.018. Epub 2006 May 15.
The lubricating abilities of different formulations of high molecular weight hyaluronic acid (HA), dipalmitoyl phosphatidylcholine (DPCC) and mixtures of both HA and DPCC were assessed in an in vitro model. Levels of start-up friction were determined using an osteoarthritis (OA) damaged human cartilage model set within a specially designed friction rig. To examine the long term benefits of HA, the extent of penetration of HA into cartilage tissue was investigated using fluorescently labelled HA and confocal microscopy. It was found that in this model, all formulations of HA and the majority of DPCC lubricants reduced friction (HA 5 and 10 mg ml(-1), DPPC 200 mg ml(-1) reductions of 51.9%, 46.7% and 46.5% respectively), compared to a Ringers solution control. Lubrication was found not to be concentration dependent for HA formulations, but concentration was key for DPCC lubrication (100 mg ml(-1) reduced friction by only 15.9%). By combining HA and DPCC (HA/DPPC; 5 mg ml(-1)/100 mg ml(-1) and 10 mg ml(-1)/200 mg ml(-1)), a further improvement was noted (69.5% and 61.9%, respectively) as the mean levels of friction were reduced by up to a further 17% than the most effective individual formulation (HA 5 mg ml(-1)). Penetration of HA into bovine cartilage by up to 300 microm from the surface was observed over a 48 h period. It was observed that HA specifically targeted the chondrocytes as it was primarily found within the lacunae surrounding the cells.
在体外模型中评估了不同配方的高分子量透明质酸（HA）、二棕榈酰磷脂酰胆碱（DPCC）以及HA与DPCC混合物的润滑能力。使用设置在专门设计的摩擦装置中的骨关节炎（OA）损伤人类软骨模型来测定起始摩擦水平。为了研究HA的长期益处，使用荧光标记的HA和共聚焦显微镜研究了HA渗透到软骨组织中的程度。结果发现，在该模型中，与林格氏液对照相比，所有HA配方以及大多数DPCC润滑剂都降低了摩擦力（HA 5和10 mg/ml，DPPC 200 mg/ml时，摩擦力分别降低了51.9%、46.7%和46.5%）。发现HA配方的润滑效果与浓度无关，但浓度是DPCC润滑的关键因素（100 mg/ml时摩擦力仅降低15.9%）。通过将HA和DPCC组合（HA/DPPC；5 mg/ml/100 mg/ml和10 mg/ml/200 mg/ml），观察到进一步的改善（分别为69.5%和61.9%），因为平均摩擦水平比最有效的单一配方（HA 5 mg/ml）又降低了17%。在48小时内观察到HA从表面向牛软骨的渗透深度可达300微米。观察到HA特异性地靶向软骨细胞，因为它主要存在于细胞周围的腔隙内。
