Rosi Nathaniel L, Giljohann David A, Thaxton C Shad, Lytton-Jean Abigail K R, Han Min Su, Mirkin Chad A
Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113 USA.
Science. 2006 May 19;312(5776):1027-30. doi: 10.1126/science.1125559.
We describe the use of gold nanoparticle-oligonucleotide complexes as intracellular gene regulation agents for the control of protein expression in cells. These oligonucleotide-modified nanoparticles have affinity constants for complementary nucleic acids that are higher than their unmodified oligonucleotide counterparts, are less susceptible to degradation by nuclease activity, exhibit greater than 99% cellular uptake, can introduce oligonucleotides at a higher effective concentration than conventional transfection agents, and are nontoxic to the cells under the conditions studied. By chemically tailoring the density of DNA bound to the surface of gold nanoparticles, we demonstrated a tunable gene knockdown.
我们描述了使用金纳米颗粒 - 寡核苷酸复合物作为细胞内基因调控剂来控制细胞中的蛋白质表达。这些寡核苷酸修饰的纳米颗粒对互补核酸的亲和常数高于未修饰的寡核苷酸对应物，不易被核酸酶活性降解，细胞摄取率大于99%，能以比传统转染剂更高的有效浓度引入寡核苷酸，并且在所研究的条件下对细胞无毒。通过化学调整结合在金纳米颗粒表面的DNA密度，我们证明了可调节的基因敲低。
