Barbet A F, Allred D R
Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville 32611-0633.
Infect Immun. 1991 Mar;59(3):971-6. doi: 10.1128/iai.59.3.971-976.1991.
A gene for the beta subunit of the immunoprotective surface antigen MSP-1 of Anaplasma marginale was previously cloned and expressed in Escherichia coli. A nucleic acid probe based on this gene detects A. marginale infection in carrier cattle and in the tick vector. We report here the sequence and structural features of the cloned msp1 beta gene and expressed polypeptide. The gene codes for a polypeptide of 756 amino acids that contains domains of tandemly repeated sequence and glutamine-rich regions at the N and C termini. The cloned copy is a member of a multigene family with multiple restriction fragment length polymorphisms in isolates of this rickettsia from different geographical regions. The availability of the sequence will allow use of the polymerase chain reaction in diagnostic assays and the preparation and testing of different vaccine constructs in cattle.
边缘无形体免疫保护性表面抗原MSP-1的β亚基基因先前已被克隆并在大肠杆菌中表达。基于该基因的核酸探针可检测携带牛和蜱传载体中的边缘无形体感染。我们在此报告克隆的msp1β基因和表达多肽的序列及结构特征。该基因编码一个由756个氨基酸组成的多肽，其在N和C末端含有串联重复序列结构域和富含谷氨酰胺的区域。克隆的拷贝是一个多基因家族的成员，在来自不同地理区域的这种立克次氏体分离株中具有多个限制性片段长度多态性。该序列的可用性将允许在诊断检测中使用聚合酶链反应，并用于牛中不同疫苗构建体的制备和测试。
