Ogawa T, Nishimura I
The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, CA 90095-1668, USA.
J Dent Res. 2006 Jun;85(6):566-70. doi: 10.1177/154405910608500617.
Bone generation occurs around titanium implants; however, its underlying mechanisms are unknown. We hypothesized that molecular determinants distinct from those undertaking normal bone healing regulate osseointegration. Using differential display-polymerase chain-reaction in the male rat model, we isolated 3 genes that are differentially expressed in bone healing with implants, but not in osteotomy healing. A homology search indicated that these 3 genes are apolipoprotein E, prolyl 4-hydroxylase alpha-subunit, and an unknown transcript. Differential expression of these genes was remarkable during early healing stages up to week 2, and accelerated with rough acid-etched surfaces compared with machined surfaces. The differential expression was confirmed in the female rats, with enhanced expression for the acid-etched surfaces. The osseointegration-unfavorable condition created by gonadal estrogen deficiency reduced the level of differential expression. This study provides evidence that selected gene transcripts are induced by titanium implants under regulatory control strongly associated with the nature of osseointegration.
钛植入物周围会发生骨生成；然而，其潜在机制尚不清楚。我们推测，与正常骨愈合过程中起作用的分子决定因素不同的分子决定因素会调节骨整合。在雄性大鼠模型中使用差异显示聚合酶链反应，我们分离出了3个在植入物骨愈合过程中差异表达，但在截骨愈合过程中不表达的基因。同源性搜索表明，这3个基因分别是载脂蛋白E、脯氨酰4-羟化酶α亚基和一个未知转录本。这些基因在愈合早期直至第2周的差异表达显著，与机械加工表面相比，粗糙酸蚀表面的差异表达加速。在雌性大鼠中也证实了这种差异表达，酸蚀表面的表达增强。性腺雌激素缺乏导致的不利于骨整合的条件降低了差异表达水平。这项研究提供了证据，表明选定的基因转录本是由钛植入物诱导产生的，且受到与骨整合性质密切相关的调控。
