蛙脑促黑素释放抑制因子（黑素抑制素）的特性：与人类神经肽Y的同源性
Characterization of melanotropin-release-inhibiting factor (melanostatin) from frog brain: homology with human neuropeptide Y.
作者信息
Chartrel N, Conlon J M, Danger J M, Fournier A, Tonon M C, Vaudry H
机构信息
European Institute for Peptide Research, Laboratory of Molecular Endocrinology, Centre National de la Recherche Scientifique, URA 650, Mont-Saint-Aignan, France.
出版信息
Proc Natl Acad Sci U S A. 1991 May 1;88(9):3862-6. doi: 10.1073/pnas.88.9.3862.
Abstract
A polypeptide was purified from frog brain extracts on the basis of its ability to inhibit alpha-melanotropin release from perifused frog neurointermediate lobes. Based on Edman degradation, amino acid analysis, and peptide mapping, the primary structure of this frog melanotropin-release-inhibiting factor (melanostatin) was determined to be H-Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Met- Ala-Lys-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg- Tyr-NH2 . Frog melanostatin belongs to the pancreatic polypeptide/neuropeptide Y/peptide YY family, and the structure of this peptide differs from that of human neuropeptide Y by only one amino acid substitution in position 19. A synthetic replicate of frog melanostatin is coeluted with the native peptide on HPLC and is highly potent in inhibiting alpha-melanotropin secretion in vitro (IC50 = 60 nM).
摘要
一种多肽是从蛙脑提取物中纯化出来的，其依据是它能够抑制从经灌流的蛙神经中间叶释放α-促黑素。基于埃德曼降解法、氨基酸分析和肽图谱分析，确定这种蛙促黑素释放抑制因子（黑素抑制素）的一级结构为H-Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Met-Ala-Lys-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr-NH2。蛙黑素抑制素属于胰多肽/神经肽Y/肽YY家族，并且该肽的结构与人类神经肽Y的结构仅在第19位有一个氨基酸取代不同。蛙黑素抑制素的合成复制品在高效液相色谱上与天然肽共洗脱，并且在体外抑制α-促黑素分泌方面具有高效力（IC50 = 60 nM）。
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本文引用的文献
1
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Proc Natl Acad Sci U S A. 1982 Apr;79(8):2514-8. doi: 10.1073/pnas.79.8.2514.
2
Growth hormone-releasing factor from a human pancreatic tumor that caused acromegaly.来自导致肢端肥大症的人类胰腺肿瘤的生长激素释放因子。
Science. 1982 Nov 5;218(4572):585-7. doi: 10.1126/science.6812220.
3
Cloning, characterization, and DNA sequence of a human cDNA encoding neuropeptide tyrosine.
Proc Natl Acad Sci U S A. 1984 Jul;81(14):4577-81. doi: 10.1073/pnas.81.14.4577.
4
Purification and characterization of human neuropeptide Y from adrenal-medullary phaeochromocytoma tissue.从肾上腺髓质嗜铬细胞瘤组织中纯化和鉴定人神经肽Y
Biochem J. 1984 May 1;219(3):699-706. doi: 10.1042/bj2190699.
5
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Endocrinology. 1983 Jan;112(1):133-41. doi: 10.1210/endo-112-1-133.
6
Rapid analysis of amino acids using pre-column derivatization.采用柱前衍生化法快速分析氨基酸。
J Chromatogr. 1984 Dec 7;336(1):93-104. doi: 10.1016/s0378-4347(00)85133-6.
7
Characterization of a growth hormone-releasing factor from a human pancreatic islet tumour.来自人胰岛肿瘤的生长激素释放因子的特性鉴定
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8
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9
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10
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