Delgado Osvaldo, Quillaguamán Jorge, Bakhtiar Shahrzad, Mattiasson Bo, Gessesse Amare, Hatti-Kaul Rajni
Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Int J Syst Evol Microbiol. 2006 Jun;56(Pt 6):1229-1232. doi: 10.1099/ijs.0.63633-0.
Strain DSM 17733(T), isolated from the shore of Lake Abjata in Ethiopia, is a heterotrophic, alkaliphilic, moderately halophilic, Gram-positive, strictly aerobic, non-motile,non-endospore-forming bacterium. The organism grows optimally at 30-37 degrees C, pH 9 and 3 % (w/v) NaCl. Analysis of the cell wall showed the presence of murein of the type L-lys-gly-L-Glu, variation A4alpha. The G + C content of the genomic DNA was 69.0 mol%. Sequence analysis of 16S rRNA gene sequence of strain DSM 17733(T) placed the isolate in the genus Nesterenkonia. DNA-DNA hybridization of DSM 17733(T) with those organisms with the closest phylogenetic affiliation, i.e. Nesterenkonia halobia, Nesterenkonia lacusekhoensis and Nesterenkonia xinjiangensis, gave relatedness values of 48.5 %, 63.7 % (repetition, 57.2 %) and 35.7 % (repetition, 29.3 %), respectively. On the basis of both phenotypic and phylogenetic criteria and the low levels of DNA-DNA relatedness with the phylogenetically closest species N. xinjiangensis and N. halobia, it is proposed that the isolate be classified in a novel species, Nesterenkonia aethiopica sp. nov. The type strain is DSM 17733(T) (=CCUG 48939(T)).
菌株DSM 17733(T) 从埃塞俄比亚的阿巴亚塔湖岸边分离得到，是一种异养、嗜碱、中度嗜盐、革兰氏阳性、严格需氧、不运动、不形成芽孢的细菌。该菌在30-37℃、pH 9和3%（w/v）NaCl条件下生长最佳。细胞壁分析表明存在L-lys-gly-L-Glu型的胞壁质，变异类型为A4α。基因组DNA的G + C含量为69.0 mol%。菌株DSM 17733(T) 的16S rRNA基因序列分析将该分离株归入涅斯捷连科氏菌属。DSM 17733(T) 与亲缘关系最近的生物即嗜盐涅斯捷连科氏菌、拉克索涅斯捷连科氏菌和新疆涅斯捷连科氏菌进行DNA-DNA杂交，其相关性值分别为48.5%、63.7%（重复，57.2%）和35.7%（重复，29.3%）。基于表型和系统发育标准以及与系统发育关系最密切的物种新疆涅斯捷连科氏菌和嗜盐涅斯捷连科氏菌的低水平DNA-DNA相关性，建议将该分离株分类为一个新物种，即埃塞俄比亚涅斯捷连科氏菌（Nesterenkonia aethiopica sp. nov.）。模式菌株为DSM 17733(T)（=CCUG 48939(T)）。
