Fernandes C, Lalitha V S, Rao K V
Carcinogenesis Division, Tata Memorial Centre, Parel, Bombay, India.
Carcinogenesis. 1991 May;12(5):839-45. doi: 10.1093/carcin/12.5.839.
The effects of malachite green (MG) and phenobarbitone (PB) were compared on the development of pre-neoplastic lesions during N-nitrosodiethylamine(DEN)-induced hepatocarcinogenesis in male Wistar rats. Rats were administered 200 p.p.m. DEN in drinking water for a period of 1 month. After an interval of 2 weeks the animals were given either MG (25 p.p.m.) or PB (500 p.p.m.) in drinking water for 2.5 months. The effects were monitored on the basis of the morphological appearance of the liver, histological pattern, gamma-glutamyltranspeptidase (GGT)-positive foci, total GGT activity and the induction of glycogen-deficient islands. Both MG and PB were found to enhance liver carcinogenesis to a significant extent when compared with either their corresponding controls or animals given DEN alone. The enhancing effect of MG at 25 p.p.m. is comparable with PB at 500 p.p.m. An enhancing effect of MG on DEN-induced hepatocarcinogenesis in the rats was demonstrated.
比较了孔雀石绿（MG）和苯巴比妥（PB）对雄性Wistar大鼠在N-亚硝基二乙胺（DEN）诱导的肝癌发生过程中癌前病变发展的影响。给大鼠饮用含200 ppm DEN的水，持续1个月。间隔2周后，给动物饮用含MG（25 ppm）或PB（500 ppm）的水，持续2.5个月。根据肝脏的形态外观、组织学模式、γ-谷氨酰转肽酶（GGT）阳性灶、总GGT活性以及糖原缺乏岛的诱导情况来监测效果。与相应对照组或仅给予DEN的动物相比，发现MG和PB均能在很大程度上增强肝癌发生。25 ppm的MG增强作用与500 ppm的PB相当。证实了MG对大鼠DEN诱导的肝癌发生有增强作用。
