Wang Qianqian, Qin Xiaofei, Geng Liping, Wang Yan
Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China.
Nanomaterials (Basel). 2019 Feb 8;9(2):229. doi: 10.3390/nano9020229.
A label-free electrochemical aptasensor was fabricated to sensitively determine malachite green (MG) based on Au nanoparticles/graphene quantum dots-tungsten disulfide nanosheet composite film modified glassy carbon electrode (AuNPs/GQDs-WS₂/GCE). A facial strategy for the self-assembly of graphene quantum dots (GQDs) on tungsten disulfide nanosheets (WS₂) was developed to fabricate 0D/2D nanocomposites. As-prepared GQDs-WS₂ hybrids exhibited significantly enhanced electrocatalytic properties, and were first used as electroactive materials to construct electrochemical aptasensor. The AuNPs/GQDs-WS₂/GCE was prepared through depositing Au nanoparticles on the surface of the GQDs-WS₂ film, which acted as the electrochemical sensing matrix to covalently immobilize the aptamers of MG via the Au⁻S bond. In this label-free proposal, the aptasensor was applied to detect MG by monitoring voltammetric signal resulted from electrochemical oxidation of the MG captured by the aptamer. Under the optimized conditions, the aptasensor showed a wide linear range from 0.01 to 10 μM for MG detection with a low detection limit of 3.38 nM (S/N = 3). The method was applied to determination of MG in spiked fish samples and gave satisfactory results.
基于金纳米颗粒/石墨烯量子点-二硫化钨纳米片复合膜修饰玻碳电极（AuNPs/GQDs-WS₂/GCE）制备了一种无标记电化学适体传感器，用于灵敏检测孔雀石绿（MG）。开发了一种在二硫化钨纳米片（WS₂）上自组装石墨烯量子点（GQDs）的简便策略，以制备0D/2D纳米复合材料。所制备的GQDs-WS₂杂化物表现出显著增强的电催化性能，并首次用作电活性材料构建电化学适体传感器。AuNPs/GQDs-WS₂/GCE是通过在GQDs-WS₂膜表面沉积金纳米颗粒制备的，该膜作为电化学传感基质，通过Au⁻S键共价固定MG的适体。在这种无标记方案中，适体传感器通过监测由适体捕获的MG电化学氧化产生的伏安信号来检测MG。在优化条件下，该适体传感器对MG检测的线性范围为0.01至10 μM，检测限低至3.38 nM（S/N = 3）。该方法应用于加标鱼样中MG的测定，结果令人满意。
