Quantification of in vitro and in vivo Cryptosporidium parvum infection by using real-time PCR.
作者信息
Godiwala Nihal T, Vandewalle Alain, Ward Honorine D, Leav Brett A
机构信息
Division of Geographic Medicine and Infectious Diseases, Tufts-New England Medical Center, Box 041, 750 Washington Street, Boston, MA 02111, USA.
出版信息
Appl Environ Microbiol. 2006 Jun;72(6):4484-8. doi: 10.1128/AEM.00189-06.
Abstract
Established methods for quantifying experimental Cryptosporidium infection are highly variable and subjective. We describe a new technique using quantitative real-time PCR (qPCR) that can be used to measure in vitro and in vivo laboratory infections with Cryptosporidium. We show for the first time that qPCR permits absolute quantification of the parasite while simultaneously controlling for the amount of host tissue and correlates significantly with established methods of quantification in in vitro and in vivo laboratory models of infection.
摘要
相似文献
1
Quantification of in vitro and in vivo Cryptosporidium parvum infection by using real-time PCR.
Appl Environ Microbiol. 2006 Jun;72(6):4484-8. doi: 10.1128/AEM.00189-06.
2
Two novel genomic DNA sequences as common diagnostic targets to detect and : Development of quantitative polymerase chain reaction assays, and clinical evaluation.
Indian J Med Microbiol. 2020 Jul-Dec;38(3 & 4):430-439. doi: 10.4103/ijmm.IJMM_20_114.
3
Specific and quantitative detection and identification of Cryptosporidium hominis and C. parvum in clinical and environmental samples.
Exp Parasitol. 2013 Sep;135(1):142-7. doi: 10.1016/j.exppara.2013.06.014. Epub 2013 Jul 6.
4
Cryptosporidium Diagnostic Assays: Molecular Detection.
Methods Mol Biol. 2020;2052:11-22. doi: 10.1007/978-1-4939-9748-0_2.
5
Comparative sensitivity of PCR primer sets for detection of Cryptosporidium parvum.
Korean J Parasitol. 2009 Sep;47(3):293-7. doi: 10.3347/kjp.2009.47.3.293. Epub 2009 Aug 28.
6
First record of Cryptosporidium infection in a raccoon dog (Nyctereutes procyonoides viverrinus).
Vet Parasitol. 2004 Mar 25;120(3):171-5. doi: 10.1016/j.vetpar.2004.01.007.
7
Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR.
Parasit Vectors. 2019 Dec 19;12(1):596. doi: 10.1186/s13071-019-3851-7.
8
Characterisation of a Cryptosporidium parvum-specific cDNA clone and detection of parasite DNA in mucosal scrapings of infected mice.
Mol Biochem Parasitol. 1998 Sep 1;95(1):21-31. doi: 10.1016/s0166-6851(98)00063-2.
9
Differentiating human from animal isolates of Cryptosporidium parvum.
Emerg Infect Dis. 1998 Oct-Dec;4(4):681-5. doi: 10.3201/eid0404.980424.
10
Detection of Cryptosporidium parvum in environmental soil and vegetables.
J Korean Med Sci. 2014 Oct;29(10):1367-71. doi: 10.3346/jkms.2014.29.10.1367. Epub 2014 Oct 8.
引用本文的文献
1
Assessment of the potential occurrence of species in various water sources in Sharqia Governorate, Egypt.
J Parasit Dis. 2024 Jun;48(2):358-369. doi: 10.1007/s12639-024-01675-1. Epub 2024 May 9.
2
The Marine Compound Tartrolon E Targets the Asexual and Early Sexual Stages of .
Microorganisms. 2022 Nov 15;10(11):2260. doi: 10.3390/microorganisms10112260.
3
Dual Transcriptomics To Determine Gamma Interferon-Independent Host Response to Intestinal Cryptosporidium parvum Infection.
Infect Immun. 2022 Feb 17;90(2):e0063821. doi: 10.1128/iai.00638-21. Epub 2021 Dec 20.
4
Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis of spp. in Stool Samples.
Pathogens. 2021 Sep 2;10(9):1131. doi: 10.3390/pathogens10091131.
5
Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR.
Parasit Vectors. 2019 Dec 19;12(1):596. doi: 10.1186/s13071-019-3851-7.
6
Interferon-λ3 Promotes Epithelial Defense and Barrier Function Against Cryptosporidium parvum Infection.
Cell Mol Gastroenterol Hepatol. 2019;8(1):1-20. doi: 10.1016/j.jcmgh.2019.02.007. Epub 2019 Mar 5.
7
In vitro determination of anticryptosporidial activity of phytogenic extracts and compounds.
Parasitol Res. 2012 Jul;111(1):231-40. doi: 10.1007/s00436-012-2824-y. Epub 2012 Jan 26.
8
A screening pipeline for antiparasitic agents targeting cryptosporidium inosine monophosphate dehydrogenase.
PLoS Negl Trop Dis. 2010 Aug 10;4(8):e794. doi: 10.1371/journal.pntd.0000794.
9
Aged HCT-8 cell monolayers support Cryptosporidium parvum infection.
Appl Environ Microbiol. 2007 Dec;73(23):7548-51. doi: 10.1128/AEM.01579-07. Epub 2007 Oct 12.
本文引用的文献
1
An early intestinal mucosal source of gamma interferon is associated with resistance to and control of Cryptosporidium parvum infection in mice.
Infect Immun. 2005 Dec;73(12):8425-8. doi: 10.1128/IAI.73.12.8425-8428.2005.
2
Application of quantitative real-time reverse transcription-PCR in assessing drug efficacy against the intracellular pathogen Cryptosporidium parvum in vitro.
Antimicrob Agents Chemother. 2005 Nov;49(11):4437-42. doi: 10.1128/AAC.49.11.4437-4442.2005.
3
Environmental temperature controls Cryptosporidium oocyst metabolic rate and associated retention of infectivity.
Appl Environ Microbiol. 2005 Jul;71(7):3848-57. doi: 10.1128/AEM.71.7.3848-3857.2005.
4
Quantitative-PCR assessment of Cryptosporidium parvum cell culture infection.
Appl Environ Microbiol. 2005 Mar;71(3):1495-500. doi: 10.1128/AEM.71.3.1495-1500.2005.
5
Surveillance for waterborne-disease outbreaks associated with recreational water--United States, 2001-2002.
MMWR Surveill Summ. 2004 Oct 22;53(8):1-22.
6
Borrelia burgdorferi-induced expression of matrix metalloproteinases from human chondrocytes requires mitogen-activated protein kinase and Janus kinase/signal transducer and activator of transcription signaling pathways.
Infect Immun. 2004 May;72(5):2864-71. doi: 10.1128/IAI.72.5.2864-2871.2004.
7
Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR.
J Clin Microbiol. 2004 Mar;42(3):1220-3. doi: 10.1128/JCM.42.3.1220-1223.2004.
8
Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage.
Appl Environ Microbiol. 2003 Sep;69(9):5178-85. doi: 10.1128/AEM.69.9.5178-5185.2003.
9
Cell culture-Taqman PCR assay for evaluation of Cryptosporidium parvum disinfection.
Appl Environ Microbiol. 2003 May;69(5):2505-11. doi: 10.1128/AEM.69.5.2505-2511.2003.
10
An immunomagnetic separation-real-time PCR method for quantification of Cryptosporidium parvum in water samples.
J Microbiol Methods. 2003 Jul;54(1):29-36. doi: 10.1016/s0167-7012(03)00005-8.
Zotero 插件