Papa Anna, Bino Silva, Velo Enkelejda, Harxhi Arjan, Kota Majlinda, Antoniadis Antonis
A' Department of Microbiology, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
J Clin Virol. 2006 Aug;36(4):272-6. doi: 10.1016/j.jcv.2006.04.007. Epub 2006 Jun 9.
Crimean-Congo hemorrhagic fever (CCHF) virus causes severe disease in humans with a mortality reaching 30%. A CCHF outbreak took place in Albania in 2003. As in other viral hemorrhagic fevers cytokines may be involved and play a role in the pathogenesis and outcome of the disease.
To investigate the levels of TNF-alpha, sTNF-R, IL-6 and IL-10 in serum samples obtained from laboratory confirmed CCHF cases and relate them to the severity of the disease.
A study population of 51 was divided into three groups: group A, consisting of PCR-positive cases; group B, consisting of PCR-negative and serology-positive cases; group C, consisting of doubly negative cases. Concentrations of serum TNF-alpha, sTNF-R, IL-6 and IL-10 were measured during the illness.
High levels of all cytokines tested were present in one fatal case. Statistically significant differences between the groups were obtained for TNF-alpha and IL-6: TNF-alpha was detected in 3 cases in group A, and in none of the other groups, while IL-6 was elevated in 10/16 patients in group A, 4/9 in group B, and 4/26 in group C. sTNF-R was not significantly different for the three groups. High concentration of IL-10 was detected only in the fatal case.
TNF-alpha and IL-6 are the cytokines most often detected during a CCHF viral infection. TNF-alpha was associated with the severe form of CCHF, while IL-6 was elevated in both severe and mild cases.
克里米亚-刚果出血热（CCHF）病毒可导致人类患上严重疾病，死亡率达30%。2003年阿尔巴尼亚爆发了克里米亚-刚果出血热疫情。与其他病毒性出血热一样，细胞因子可能参与其中，并在该疾病的发病机制及转归中发挥作用。
研究实验室确诊的克里米亚-刚果出血热病例血清样本中肿瘤坏死因子-α（TNF-α）、可溶性肿瘤坏死因子受体（sTNF-R）、白细胞介素-6（IL-6）和白细胞介素-10（IL-10）的水平，并将其与疾病严重程度相关联。
51名研究对象被分为三组：A组，由聚合酶链反应（PCR）阳性病例组成；B组，由PCR阴性且血清学阳性病例组成；C组，由双阴性病例组成。在患病期间检测血清TNF-α、sTNF-R、IL-6和IL-10的浓度。
在一例死亡病例中检测到所有受试细胞因子水平均较高。TNF-α和IL-6在各组间存在统计学显著差异：A组3例检测到TNF-α，其他组均未检测到；而IL-6在A组16例患者中有10例升高，B组9例中有4例升高，C组26例中有4例升高。三组间sTNF-R无显著差异。仅在死亡病例中检测到高浓度的IL-10。
TNF-α和IL-6是克里米亚-刚果出血热病毒感染期间最常检测到的细胞因子。TNF-α与克里米亚-刚果出血热的严重形式相关，而IL-6在重症和轻症病例中均升高。
