Zhang Ying, Li Hai-Zhou, Zhang Ying-Jun, Jacob Melissa R, Khan Shabana I, Li Xing-Cong, Yang Chong-Ren
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, PR China
Steroids. 2006 Aug;71(8):712-9. doi: 10.1016/j.steroids.2006.04.005.
Atropurosides A-G (1-7), seven new steroidal saponins, which possess new polyhydroxylated aglycones, were isolated from the rhizomes of Smilacina atropurpurea (Convallariaceae), together with a known saponin, dioscin (8). Their structures were elucidated on the basis of detailed spectroscopic analysis, including 1D and 2D NMR techniques and chemical methods. Antifungal testing of the eight compounds indicated that atropurosides B (2) and F (6) were fungicidal against Candida albicans, Candida glabrata, Cryptococcus neoformans, and Aspergillus fumigatus with minimum fungicidal concentrations (MFCs) < or = 20 microg/ml, while dioscin (8) was selectively active against C. albicans and C. glabrata (MFC < or = 5.0 microg/ml). Furthermore, the antifungal saponins 2, 6, and 8 were evaluated for their in vitro cytotoxicities in a panel of human cancer cell lines (SK-MEL, KB, BT-549, SK-OV-3, and HepG2) and non-cancerous Vero cells. All showed moderate cytotoxicities. It appears that the antifungal activity of these steroidal saponins correlates with their cytotoxicity against mammalian cells.
从紫花鹿药（菝葜科）的根茎中分离得到了7个新的甾体皂苷阿托普皂苷A-G（1-7），它们具有新的多羟基苷元，同时还得到了一个已知的皂苷薯蓣皂苷（8）。基于详细的光谱分析，包括一维和二维核磁共振技术以及化学方法，阐明了它们的结构。对这8种化合物的抗真菌测试表明，阿托普皂苷B（2）和F（6）对白色念珠菌、光滑念珠菌、新生隐球菌和烟曲霉具有杀菌活性，其最低杀菌浓度（MFC）≤20μg/ml，而薯蓣皂苷（8）对白色念珠菌和光滑念珠菌具有选择性活性（MFC≤5.0μg/ml）。此外，还对具有抗真菌活性的皂苷2、6和8在一组人癌细胞系（SK-MEL、KB、BT-549、SK-OV-3和HepG2）和非癌细胞Vero细胞中进行了体外细胞毒性评估。所有化合物均表现出中等细胞毒性。这些甾体皂苷的抗真菌活性似乎与其对哺乳动物细胞的细胞毒性相关。
