Minamizawa Kiyoshi, Goto Hirozo, Shimada Yutaka, Terasawa Katsutoshi, Haji Akira
Department of Japanese Oriental Medicine, Faculty of Medicine, University of Toyama, Japan.
J Pharmacol Sci. 2006 Jun;101(2):118-25. doi: 10.1254/jphs.fp0060135. Epub 2006 Jun 15.
To evaluate the efficacy of three common antitussive Kampo formulas, eppikahangeto (EPP), bakumondoto (BAK), and shoseiryutogomakyokansekito (SGM), a new cough model of guinea pig was used, which could specifically induce a laryngeal cough by microinjection of citric acid solution into the larynx. Kampo extract was dissolved in water and the animals were given access ad libitum for 3 days, and then the number of coughs during 10 min was counted. EPP extract decreased the number of coughs dose-dependently (0.3% extract, -22.9 +/- 6.6%, P<0.01; 1.0% extract, -32.4 +/- 5.5%, P<0.01). BAK extract and SGM extract had no significant effect. Intraperitoneal injection of codeine (60 mg/kg) also decreased the number of coughs (-36.1 +/- 9.1%, P<0.05). Furthermore, Ephedrae herba (EH) extract reduced the number of coughs (-18.3 +/- 6.0%, P<0.05), but the extract of EPP without EH did not. These results suggest that EPP has an antitussive effect against laryngeally-induced cough in guinea pigs, and the crucial herbal medicine is EH.
为评估三种常见的汉方止咳方剂，即麻黄汤（EPP）、麦门冬汤（BAK）和小青龙加石膏汤（SGM）的疗效，使用了一种新的豚鼠咳嗽模型，该模型可通过向喉部微量注射柠檬酸溶液特异性诱发喉源性咳嗽。将汉方提取物溶于水，让动物自由饮用3天，然后计算10分钟内的咳嗽次数。EPP提取物可剂量依赖性地减少咳嗽次数（0.3%提取物，-22.9±6.6%，P<0.01；1.0%提取物，-32.4±5.5%，P<0.01）。BAK提取物和SGM提取物无显著效果。腹腔注射可待因（60mg/kg）也可减少咳嗽次数（-36.1±9.1%，P<0.05）。此外，麻黄（EH）提取物可减少咳嗽次数（-18.3±6.0%，P<0.05），但不含EH的EPP提取物则无此作用。这些结果表明，EPP对豚鼠喉源性咳嗽具有止咳作用，关键草药为EH。
