Jackson J K, Higo T, Hunter W L, Burt H M
Faculty of Pharmaceutical Sciences, University of British Columbia, 2146 East Mall, Vancouver BC, Canada V6T 1Z3.
Inflamm Res. 2006 Apr;55(4):168-75. doi: 10.1007/s00011-006-0067-z.
Curcumin and quercetin are antioxidant molecules with anti-proliferative, anti-inflammatory and immunosuppressive activities. The objective of this study was to investigate the inhibitory activity of these agents using four assays of inflammatory aspects of arthritis.
Crystal-induced neutrophil activation was measured by luminol-dependent chemiluminescence. Synoviocyte proliferation was measured by an MTS assay using HIG-82 rabbit synoviocytes in cell culture. Chondrocyte (cultured primary cells) expression of the matrix metalloproteinases collagenase and stromelysin was measured by Northern Blot analysis. Angiogenesis was measured using the chorioallantoic membrane of the chick embryo.
Both agents inhibited neutrophil activation, synoviocyte proliferation and angiogenesis. Curcumin strongly inhibited collagenase and stromelysin expression at micromolar concentrations whereas quercetin had no effect in this assay.
These studies suggest that curcumin and to a lesser extent quercetin may offer therapeutic potential for the treatment of crystal-induced arthritis or rheumatoid arthritis.
姜黄素和槲皮素是具有抗增殖、抗炎和免疫抑制活性的抗氧化分子。本研究的目的是通过四种关节炎炎症方面的检测方法来研究这些药物的抑制活性。
通过鲁米诺依赖性化学发光法测定晶体诱导的中性粒细胞活化。使用细胞培养中的HIG-82兔滑膜细胞，通过MTS检测法测定滑膜细胞增殖。通过Northern印迹分析测定软骨细胞（培养的原代细胞）中基质金属蛋白酶胶原酶和基质溶解素的表达。使用鸡胚绒毛尿囊膜测定血管生成。
两种药物均抑制中性粒细胞活化、滑膜细胞增殖和血管生成。姜黄素在微摩尔浓度下强烈抑制胶原酶和基质溶解素的表达，而槲皮素在该检测中无作用。
这些研究表明，姜黄素以及程度稍轻的槲皮素可能为晶体诱导性关节炎或类风湿性关节炎的治疗提供治疗潜力。
