Ugryumova Nadezhda, Gangnus Sergei V, Matcher Stephen J
Biomedical Physics Group, University of Exeter, Stocker Road, Exeter EX4 4QL, UK.
Opt Lett. 2006 Aug 1;31(15):2305-7. doi: 10.1364/ol.31.002305.
Polarization optical coherence tomography (PSOCT) is a powerful technique to nondestructively map the retardance and fast-axis orientation of birefringent biological tissues. Previous studies have concentrated on the case where the optic axis lies on the plane of the surface. We describe a method to determine the polar angle of the optic axis of a uniaxial birefringent tissue by making PSOCT measurements with a number of incident illumination directions. The method is validated on equine flexor tendon, yielding a variability of 4% for the true birefringence and 3% for the polar angle. We use the method to map the polar angle of fibers in the transitional region of equine cartilage.
偏振光学相干断层扫描(PSOCT)是一种用于无损测绘双折射生物组织的延迟和快轴方向的强大技术。以往的研究主要集中在光轴位于表面平面的情况。我们描述了一种通过使用多个入射照明方向进行PSOCT测量来确定单轴双折射组织光轴极角的方法。该方法在马屈肌腱上得到验证,真双折射的变异性为4%,极角的变异性为3%。我们使用该方法测绘马软骨过渡区域纤维的极角。