Bingen E H, Denamur E, Lambert-Zechovsky N Y, Elion J
Laboratoire de Microbiologie, Hôpital Robert Debré, Paris, France.
J Clin Microbiol. 1991 Sep;29(9):1888-92. doi: 10.1128/jcm.29.9.1888-1892.1991.
Vancomycin-resistant enterococci are now found with increasing frequency. Up to now, epidemiological studies of enterococci have been limited by the lack of convenient and accessible methods for comparing strains. In this study, we report an epidemiological investigation on 16 nosocomial vancomycin-resistant Enterococcus faecium strains isolated from 15 patients in four different wards of a children's hospital over a period of 17 months. Analysis of restriction fragment length polymorphism (RFLP) of total DNA and of ribosomal DNA regions (ribotyping) was used as a typing approach. Each strain produced a different total DNA RFLP pattern after HindIII and PvuII digestion, except for two strains that were isolated from a single patient and that gave indistinguishable patterns. In our system, ribotyping was less discriminative than RFLP of total DNA. This approach, therefore, shows the genetic unrelatedness of the nosocomial strains studied and excludes patient-to-patient strain transmission either in the same ward or between wards.
耐万古霉素肠球菌的发现频率日益增加。到目前为止，肠球菌的流行病学研究因缺乏方便且可及的菌株比较方法而受到限制。在本研究中，我们报告了一项对16株耐万古霉素屎肠球菌进行的流行病学调查，这些菌株是在17个月的时间里从一家儿童医院四个不同病房的15名患者中分离得到的。对总DNA的限制性片段长度多态性（RFLP）分析以及核糖体DNA区域分析（核糖体分型）被用作分型方法。除了从一名患者身上分离出的两株菌株呈现出无法区分的图谱外，每株菌株在经HindIII和PvuII消化后均产生了不同的总DNA RFLP图谱。在我们的系统中，核糖体分型的鉴别能力不如总DNA的RFLP。因此，该方法表明所研究的医院菌株在基因上没有相关性，排除了同一病房或不同病房之间患者与患者之间的菌株传播。
