Tang Y W, Li Y L, Ye K L, Xu Z Y, Ruo S L, Fisher-Hoch S P, McCormick J B
Department of Epidemiology, School of Public Health, Shanghai Medical University, People's Republic of China.
J Clin Microbiol. 1991 Sep;29(9):1924-7. doi: 10.1128/jcm.29.9.1924-1927.1991.
An epidemiologic evaluation of patients with hemorrhagic fever with renal syndrome from different locations in the People's Republic of China was conducted to define the prevalence of two Hantavirus serotypes, Seoul (SEO) and Hantaan (HTN). Serum specimens were collected between 5 and 14 days after the onset of illness and were tested for antibodies by both hemagglutination inhibition (HI) and plaque reduction neutralization (PRN). By the HI test, the geometric mean titer (GMT) of antibodies to SEO in the sera from individuals from Kaifeng City of Henan Province was five times higher than that to HTN. In contrast, by the HI test, the sera from individuals from Jiande County of Zhejiang Province had a GMT of antibodies to HTN that was seven times higher than that to SEO. In the sera from individuals from Shanghai, only a twofold difference was observed in HI antibody titers to the two hemagglutinins by the HI test, with that to HTN being higher than that to SEO. By the PRN test, the GMT ratios of antibody between HTN and SEO strains from individuals in Kaifeng, Jiande, and Shanghai were found to be 1:13, 14:1, and 2:1 respectively. A close correlation (r = 0.8219) and concordance rate (78.3%) were observed between the PRN and HI tests for the identification of the serotypes of individual cases of hemorrhagic fever with renal syndrome. The hantavirus serotypes from individuals in Kaifeng and Jiande were identified as predominantly SEO and HTN, respectively, and those from individuals in Shanghai had an indeterminant serotype defined by these two techniques. The HI test appears to be a simple and reliable way of determining the predominant hantavirus that causes HFRS in a given geographic area.
对中华人民共和国不同地区的肾综合征出血热患者进行了一项流行病学评估，以确定两种汉坦病毒血清型，即汉城型（SEO）和汉滩型（HTN）的流行情况。在发病后5至14天收集血清标本，并通过血凝抑制（HI）和空斑减少中和（PRN）试验检测抗体。通过HI试验，河南省开封市个体血清中针对SEO的抗体几何平均滴度（GMT）比针对HTN的抗体几何平均滴度高5倍。相比之下，通过HI试验，浙江省建德县个体血清中针对HTN的抗体GMT比针对SEO的抗体GMT高7倍。在上海个体的血清中，通过HI试验观察到针对两种血凝素的HI抗体滴度仅相差两倍，针对HTN的抗体滴度高于针对SEO的抗体滴度。通过PRN试验，发现开封、建德和上海个体中针对HTN和SEO毒株的抗体GMT比值分别为1:13、14:1和2:1。在肾综合征出血热个体病例血清型鉴定中，PRN试验和HI试验之间观察到密切相关性（r = 0.8219）和符合率（78.3%）。开封和建德个体中的汉坦病毒血清型分别主要鉴定为SEO和HTN，而上海个体中的汉坦病毒血清型通过这两种技术鉴定为不确定。HI试验似乎是确定给定地理区域中导致肾综合征出血热的主要汉坦病毒的一种简单可靠的方法。
