Kurnatowski Piotr, Klimiuk Cezary, Głowacka Anna
Centre for Treatment of Parasitic Diseases and Mycoses, Medical University, Lódź.
Wiad Parazytol. 2003;49(1):61-71.
Increasing use of elctromagnetic fields (EF) in the treatment of various diseases may have potential impacts on fungi--possible aetiological factors of concomitant mycoses. The aim of this study was to investigate the effects of EF on miconazole susceptibility among fungi of the genus Candida showing confirmed pathogenicity in humans. Fifteen Candida strains obtained from patients were used and their susceptibility to miconazole was determined by diffusion in agar gel. Antifungal activity of miconazole was assessed as minimal inhibitory concentration (MIC) which was calculated for Candida strains not exposed to EF (control) and EF-exposed (3 experiments). In the majority of cases the susceptibility of Candida species to miconazole decreased (higher MICs) after the first week of EF exposure, regardless of the EF's parameters. This was followed by an increase in susceptibility (lower MICs) after the second week of exposure to EF of 2 mT intensity and frequency 3 Hz (experiment I) and an EF of 9 mT intensity and 12.5 Hz frequency (experiment II) relative to control. An increase in susceptibility (lower MICs) was observed in the second week of exposure, whatever the parameters of the EF. The application of low intensity, low frequency EF for a period of at least two weeks may be beneficial in the treatment of mycoses caused by pathogenic fungi of Candida genus.
在各种疾病治疗中越来越多地使用电磁场（EF）可能会对真菌产生潜在影响——真菌可能是伴发真菌病的病因。本研究的目的是调查电磁场对已证实对人类具有致病性的念珠菌属真菌对咪康唑敏感性的影响。使用从患者身上获得的15株念珠菌菌株，并通过琼脂凝胶扩散法测定它们对咪康唑的敏感性。将咪康唑的抗真菌活性评估为最低抑菌浓度（MIC），该浓度针对未暴露于电磁场的念珠菌菌株（对照）和暴露于电磁场的菌株（3个实验）进行计算。在大多数情况下，无论电磁场参数如何，念珠菌属对咪康唑的敏感性在暴露于电磁场的第一周后都会降低（MIC值更高）。随后，在暴露于强度为2 mT、频率为3 Hz的电磁场（实验I）以及强度为9 mT、频率为12.5 Hz的电磁场（实验II）两周后，相对于对照，敏感性增加（MIC值更低）。无论电磁场参数如何，在暴露的第二周均观察到敏感性增加（MIC值更低）。应用低强度、低频率的电磁场至少两周可能有利于治疗由念珠菌属致病真菌引起的真菌病。
