使用理查森-鲁西去卷积的薄片扫描双轴共聚焦显微镜术
Sheet-scanned dual-axis confocal microscopy using Richardson-Lucy deconvolution.
作者信息
Wang D, Meza D, Wang Y, Gao L, Liu J T C
出版信息
Opt Lett. 2014 Sep 15;39(18):5431-4. doi: 10.1364/OL.39.005431.
Abstract
We have previously developed a line-scanned dual-axis confocal (LS-DAC) microscope with subcellular resolution suitable for high-frame-rate diagnostic imaging at shallow depths. Due to the loss of confocality along one dimension, the contrast (signal-to-background ratio) of a LS-DAC microscope is deteriorated compared to a point-scanned DAC microscope. However, by using a sCMOS camera for detection, a short oblique light-sheet is imaged at each scanned position. Therefore, by scanning the light sheet in only one dimension, a thin 3D volume is imaged. Both sequential two-dimensional deconvolution and three-dimensional deconvolution are performed on the thin image volume to improve the resolution and contrast of one en face confocal image section at the center of the volume, a technique we call sheet-scanned dual-axis confocal (SS-DAC) microscopy.
摘要
我们之前开发了一种线扫描双轴共聚焦（LS-DAC）显微镜，其具有亚细胞分辨率，适用于浅深度的高帧率诊断成像。由于沿一个维度共焦性的丧失，与点扫描DAC显微镜相比，LS-DAC显微镜的对比度（信噪比）会下降。然而，通过使用sCMOS相机进行检测，在每个扫描位置对一个短的倾斜光片进行成像。因此，通过仅在一个维度上扫描光片，可以对一个薄的三维体积进行成像。对该薄图像体积进行顺序二维去卷积和三维去卷积，以提高体积中心处一个正面共焦图像切片的分辨率和对比度，我们将这种技术称为片扫描双轴共聚焦（SS-DAC）显微镜术。
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