Dwyer Peter J, DiMarzio Charles A, Rajadhyaksha Milind
Department of Electrical and Computer Engineering, Northeastern University, MA 02115, USA.
Appl Opt. 2007 Apr 1;46(10):1843-51. doi: 10.1364/ao.46.001843.
A confocal reflectance theta line-scanning microscope demonstrates imaging of nuclear and cellular morphology in human skin and oral mucosa in vivo. The illumination and detection are through a divided objective lens pupil, resulting in a theta-microscope configuration. A line is directly scanned in the pupil and descanned onto a linear detector array such that the theta line scanner consists of only seven main optical components. The experimentally measured lateral resolution is 1.0 microm and optical section thickness is 1.7 microm under nominal conditions at 830 nm wavelength. Through full-thickness human epidermis (i.e., in the dermis) the measured lateral resolution is 1.7 microm and the optical section thickness is 9.2 microm. The lateral resolution, sectioning, and image quality in epidermal (epithelial) tissue is comparable to that of point scanning confocal microscopy.
共聚焦反射θ线扫描显微镜可在体内对人体皮肤和口腔黏膜的细胞核及细胞形态进行成像。照明和检测通过分开的物镜光瞳进行，形成θ显微镜配置。一条线直接在光瞳中扫描并解扫描到线性探测器阵列上，使得θ线扫描仪仅由七个主要光学组件组成。在830nm波长的标称条件下，实验测量的横向分辨率为1.0微米，光学切片厚度为1.7微米。穿过全层人类表皮（即在真皮中）时，测量的横向分辨率为1.7微米，光学切片厚度为9.2微米。表皮（上皮）组织中的横向分辨率、切片和图像质量与点扫描共聚焦显微镜相当。
