东非和南部非洲采采蝇（Glossina morsitans morsitans Westwood）种群的遗传多样性和分化模式。
Patterns of genetic diversity and differentiation in the tsetse fly Glossina morsitans morsitans Westwood populations in East and southern Africa.
作者信息
Ouma J O, Marquez J G, Krafsur E S
机构信息
Department of Entomology, Iowa State University, Ames, Iowa 50011-3222, USA.
出版信息
Genetica. 2007 Jun;130(2):139-51. doi: 10.1007/s10709-006-9001-0. Epub 2006 Aug 4.
Genetic diversity and differentiation within and among nine G. morsitans morsitans populations from East and southern Africa was assessed by examining variation at seven microsatellite loci and a mitochondrial locus, cytochrome oxidase (COI). Mean COI diversity within populations was 0.63+/-0.33 and 0.81 taken over all populations. Diversities averaged over microsatellite loci were high (mean number of alleles/locus>or=7.4; mean HE>or=65%) in all populations. Diversities averaged across populations were greater in East Africa (mean number of alleles=22+/-2.6; mean he=0.773+/-0.033) than in southern Africa (mean number of alleles=18.7+/-4.0; mean he=0.713+/-0.072). Differentiation among all populations was highly significant (RST=0.25, FST=0.132). Nei's Gij statistics were 0.09 and 0.19 within regions for microsatellites and mitochondria, respectively; between regions, Gij was 0.14 for microsatellites and 0.23 for mitochondria. GST among populations was 0.23 for microsatellite loci and 0.40 for mitochondria. The F, G and R statistics indicate highly restricted gene flow among G. m. morsitans populations separated over geographic scales of 12-917 km.
通过检测7个微卫星位点和1个线粒体细胞色素氧化酶（COI）位点的变异，评估了来自东非和南非的9个采采蝇种群内部及种群间的遗传多样性和分化情况。种群内COI的平均多样性为0.63±0.33，所有种群的平均值为0.81。所有种群微卫星位点的平均多样性较高（平均等位基因数/位点≥7.4；平均期望杂合度≥65%）。东非种群的平均多样性（平均等位基因数=22±2.6；平均期望杂合度=0.773±0.033）高于南非种群（平均等位基因数=18.7±4.0；平均期望杂合度=0.713±0.072）。所有种群间的分化非常显著（RST=0.25，FST=0.132）。微卫星和线粒体在区域内的内氏Gij统计值分别为0.09和0.19；区域间，微卫星的Gij为0.14，线粒体的为0.23。种群间微卫星位点的GST为0.23，线粒体的为0.40。F、G和R统计值表明，在地理距离为12 - 917公里的分离采采蝇种群间，基因流动受到高度限制。
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