Pinelli Elena, Brandes Sietze, Dormans Jan, Fonville Manoj, Hamilton Clare M, der Giessen Joke van
Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
Exp Parasitol. 2007 Jan;115(1):76-82. doi: 10.1016/j.exppara.2006.06.002. Epub 2006 Sep 5.
Infection of mice with Toxocara canis results in pulmonary inflammation and the induction of a Th2 type of immune response. The aim of this study was to determine whether the effect of infection with this nematode depends on the inoculum size. Results indicate that mice infected with either a high or a low inoculum size showed, in a dose-dependent manner, pulmonary inflammation with eosinophil infiltration, increased levels of total IgE, and Toxocara-specific IgG1 that persisted up to 60 days p.i. Relative quantification of cytokine expression in lungs of mice infected with different doses showed proportional increased expression of the IL-4, IL-5, and IL-10 transcripts, whereas the expression of the IFN-gamma transcript was not different from that of uninfected controls. These results indicate that infection of BALB/c mice with T. canis results in chronic pulmonary inflammation and a dominant Th2 type of immune response, independent of the inoculum size.
用犬弓首蛔虫感染小鼠会导致肺部炎症，并诱导Th2型免疫反应。本研究的目的是确定感染这种线虫的影响是否取决于接种量。结果表明，感染高接种量或低接种量的小鼠均呈剂量依赖性地出现肺部炎症伴嗜酸性粒细胞浸润、总IgE水平升高以及犬弓首蛔虫特异性IgG1，这种情况在感染后持续长达60天。对感染不同剂量线虫的小鼠肺组织中细胞因子表达进行相对定量分析，结果显示IL-4、IL-5和IL-10转录本的表达呈比例增加，而IFN-γ转录本的表达与未感染对照组无差异。这些结果表明，用犬弓首蛔虫感染BALB/c小鼠会导致慢性肺部炎症和占主导地位的Th2型免疫反应，且与接种量无关。
