Wilcock G K, Matthews S M, Moss T
Department of Care of the Elderly, Frenchay Hospital, Bristol, Great Britain.
Acta Neuropathol. 1990;79(5):566-8. doi: 10.1007/BF00296118.
Three methods were compared to find a reliable method for demonstrating neurofibrillary tangles (NFTs) and neuritic plaques (NPs) in brain tissue stored for long periods in formalin or as paraffin blocks. The short-term fixation of tissue, e.g. up to 6 months in formalin does not usually present a problem using any of the three methods tried, e.g. Gallyas, modified Palmgren, or modified Bielschowsky, but once the time lengthens to 6 years or more the demonstration of NFTs and NPs is not so reliable using the first two methods. The modified Bielschowsky method, however, demonstrates well both NFTs and NPs in material stored in formalin or as paraffin blocks for long periods, e.g. 7 years, and also compares favourably with the other methods on freshly processed material and fixed tissue, stored for shorter periods. We also noted as a consistent trend, irrespective of the staining technique employed, the detection of fewer plaques and tangles in material stored in formalin, as opposed to that stored as blocks embeded in paraffin wax.
比较了三种方法，以找到一种可靠的方法来显示长期保存在福尔马林中或制成石蜡块的脑组织中的神经原纤维缠结（NFTs）和神经炎性斑块（NPs）。对于短期固定的组织，例如在福尔马林中固定长达6个月，使用所尝试的三种方法中的任何一种，如Gallyas法、改良的Palmgren法或改良的Bielschowsky法，通常都不会出现问题。但是，一旦时间延长到6年或更长时间，使用前两种方法显示NFTs和NPs就不那么可靠了。然而，改良的Bielschowsky法能很好地显示长期保存在福尔马林中或制成石蜡块（如保存7年）的材料中的NFTs和NPs，并且在新鲜处理的材料和短期保存的固定组织上，与其他方法相比也具有优势。我们还注意到一个一致的趋势，无论采用何种染色技术，与保存在石蜡块中的材料相比，保存在福尔马林中的材料中检测到的斑块和缠结较少。
