参与FecR结合的残基位于大肠杆菌中FecA信号结构域的一侧。
Residues involved in FecR binding are localized on one side of the FecA signaling domain in Escherichia coli.
作者信息
Breidenstein Elena, Mahren Susanne, Braun Volkmar
机构信息
Microbiology/Membrane Physiology, University of Tübingen, D-72076 Tübingen, Germany.
出版信息
J Bacteriol. 2006 Sep;188(17):6440-2. doi: 10.1128/JB.00741-06.
Abstract
Ferric citrate transport in Escherichia coli involves proteins encoded by the fec genes, including the transport and signaling protein FecA and the signal transducing protein FecR. Randomly isolated FecA point mutants showed a reduced interaction with FecR and a reduced transcription initiation of the ferric citrate transport genes. The mutations were localized on one side of the FecA signaling domain, which might form the interface to FecR. Some of the mutants showed strongly reduced iron transport rates, which suggests that the signaling domain affects the structure of the FecA transporter domain.
摘要
柠檬酸铁在大肠杆菌中的转运涉及由fec基因编码的蛋白质，包括转运和信号蛋白FecA以及信号转导蛋白FecR。随机分离的FecA点突变体与FecR的相互作用减少，柠檬酸铁转运基因的转录起始也减少。这些突变位于FecA信号结构域的一侧，该侧可能形成与FecR的界面。一些突变体的铁转运速率大幅降低，这表明信号结构域影响FecA转运结构域的结构。
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